For example, I have mml-miR-532-3p and hsa-miR-492 and I need them to be from one species. Can I consider mml-miR-532-3p the same as hsa-miR-532-3p?
Yes. If you check the targets of mature miRNA, You can see, your miR-532-3p in both species.
That means you can use this miRNA for your study both species.
İs it clear for you?
You can check this link: https://geneglobe.qiagen.com/us/product-groups/mircury-lna-mirna-pcr-assays?q=miR-532-3p
Zeynep Yılmaz Şükranlı Thanks for the answer. But I'm talking about more than just miR-532-3p. I'm talking about dozens of miRNAs. Would it be ok for all of them or are there conditions?
Many miRNAs with the same name have different sequences across species. Even if the mature miRNA sequence is identical, they could have differences in the pri-miRNA sequence which will affect which strand is loaded onto Ago. I would not do the type of analysis that you are suggesting without rigorously checking the sequences of each miRNA in both species.
- Badges
- Science method