I have to test the pathogenecity of different fungal isolates on a plant using detached leaf assay method and also seedling assay. So what would be the best option to make fungal spore suspension from PDA plate, whether to use sterile saline 0.91% or sterile distilled water? Also please tell me the possibility of storing the suspension in both cases.
Dear Arya,
I recommend the sterile saline water. But if you could use a diluted sterile PDA solution it would be much better. for example 25% of sterile PDA liquid medium. Because you don`t Know if you fungal is saline tolerant. if it is not, it can affect the fungal growth.
for storing the fungal, It is better to prepare a medium 30% glycerol-PDA based and put it under -20C.
I hope the answer brought you some help.
Regards
Badreddine Sijilmassi Thank you for the information sir.
It is Potato Dextrose broth 25% you are mentioning, right? Can I store the same spore suspension in -20C?
Thank you
The 25% PDA Broth for spore inoculation use. For storing, Normal PDA broth with 30% Glycerol at -20 C.
Good luck
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