Is it beneficial for a geneticist to have theoretical and practical courses on flow cytometry?Does it have any applications in the stem cell and regeneration technology?
Yes! FACS is indeed a useful technology in different area of cell research. It is particularly important when you deal with blood derived cell/stem cells - for sorting the cells as well as analysing the cell size and surface properties. You can read any standard book to know further details. Nonetheless, it is not absolute essential that one should certainly know how to operate a machine. Its analysis that is important. Further, it's an easy piece of cake to learn the FACS.
Sure! and we did it ,
check this!: http://www.biomedcentral.com/1746-6148/9/224
Rgds!
Hi Aamir,
I am dedicated to the study and application of stem cells in the field of cardiac regeneration. My experience with the various options that provides flow cytometry technology is largely positive, both to characterise distinct levels of surface molecules by conventional flow cytometry and to isolate different cell subpopulations by using cell sorting approaches (either wild-type or genetically modified cells for fluorescent labeling). Flow cytometry is also a great helpto predict the immunoreactivity exhibited by stem cells implanted in animal models of disease, among other purposes... but it is important to start working in collaboration with really skilled experts who show great aptitudes and availability!
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