I was reading an article, where I came across with " Thiolated DNA." How does a DTT chemical act to make a bond with the sulfur of Thiolated DNA? Please inform me about the structure of Thiolated DNA so that I understand the whole phenomenon.
Pubmed is your friend, Aamir, a quick search will tell you DNA can be modified at either extremity with a thiol group for applications in nanotechnology, specially binding them on gold particles.
http://www.ncbi.nlm.nih.gov/pubmed/?term=thiolated+dna%5Btitle%5D
It can be purchased from different brands. On what kind of paper did you find this?
Yes dear Daniel we can access papers but over here I asked so that I will get a quick answer with explanation. I was reading about Dithiothreitol where it said that "DTT is as a reducing or "deprotecting" agent for thiolated DNA, The terminal sulfur atoms of thiolated DNA have a tendency to form dimers in solution, especially in the presence of oxygen" by this I came up with the thiolated DNA. Simple answer about it and the phenomenon about this all would be nice to get and learn from an expert here.
Sigma-Aldrich has a protocol for thiolated DNA reduction with a brief explanation of the process:
http://www.sigmaaldrich.com/life-science/custom-oligos/custom-dna/learning-center/oligo-reduction.html
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