I have a problem, my plate and spacer are fused because of acrylamide and it's hard to remove I even broke one short plate. Our acrylamide is not good to use we confirmed that by an acrylamide that works very well. Any idea how to open/separate it from one another. The Acraylamide40% we used was completely new, what could be wrong in it, the rest of ingredients are perfectly fine.
May want to double.check volumes of A/P and temednd. The rehydration of gel in water should help to remove short plate. Best
An overnight soak in water should re-hydrate/over-hydrate and soften any acrylamide that is present.
Don't Put pressure at a single spot. Try and apply a short upwards thrust to all regions possible between the plates to remove the short plate. Alternately, dip your plate in running buffer and keeping the plate submerged follow the above mentioned steps. Bio-Rad plates are very delicate specially the short plate. All the best. If the problem persists do share your recipe for preparing the acrylamide solution (complete with TEMED, APS etc.) and the % resolving gel you are casting.
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