What does a negative real part of impedance indicate in a Nyquist plot?

I am trying to quantify Parylene C's hermetic encapsulation quality using EIS with a 2 electrode configuration (WE/WS and RE/CE). I am simply using two patterned gold layers on glass as my...

12 January 2021 7,399 14 View

How to clean LEDs (InGaN on SiC) and Ti/Au electrode chips?

I need perfect cleanliness for further processes but I am not sure which cleaning method would be sufficient yet letting the layers intact.

01 December 2020 1,099 2 View

What is the difference between the near field and the far field effects in optics?

A simple definition might help to understand the diffraction pattern in an aperiodic nano-micro structured surface.

19 July 2018 3,865 6 View

Is there a way to attach a nano particle to the cell membrane and prevent it from entering the cell?

01 June 2016 5,159 3 View

Will RGD coated nanoparticles be internalised by cells?

24 December 2015 6,303 4 View

Do we have "mesenchymal" breast cancer cell lines?

24 November 2015 8,669 6 View

What is the difference between various breast cancer cell lines?

24 November 2015 8,487 5 View

Is there any correlation between substrate stiffness and substrate roughness?

Can tuning roughness leads to different stiffness of a material?

18 October 2015 3,286 8 View

Is flow cytometry capable of calculating the viability rate of a low viscous cell solution?

As trypan blue fails to calculate the accurate viability rate of my [cell+alginate 1.2%] suspension, I was thinking about using dual florescent dye (PI & AO). However, I am concerned about whether...

22 February 2013 5,130 2 View

Dual-fluorescence viability VS Trypan blue, which one is more accurate and why am I observing such a distinctive difference?

While encapsulating ADMSCs in sodium alginate, providing a 3D environment for the cells, I come across different viability rates. Trypan blue shows a lower viability rate, as low as 50%. On the...

19 February 2013 6,200 5 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View