Hi, I fixed chondrocytes in 12-well plate with glutaraldehyde. I want to stain the cells by direct red. Do you have any protocol? Thank you.
I have fixed human chondrocytes in 6-well plate by glutaraldehyde. For alcian blue staining pH 1, I used this protocol: 1) Rinse wells 3x with PBS. 2) Rinse 1x with 1% acetic acid. 3) Apply...
24 October 2023 7,385 0 View
Any suggestions for high quality miRNA extraction would be appreciated.
13 June 2023 5,958 3 View
Please help me. I don't know how can I design laparoscope with zemax.
01 April 2023 3,150 0 View
I use the typical syringe pumps to load organoids (200-300 um in diameter) into my device; however, I don't see them in the chip! I swapped them with beads (similar size) instead and observed that...
26 February 2023 944 2 View
It's quite confusing to distinguish between the definitions. To me, autofluorescence fits all. But what is the correct terminology to use when talking about biological cells, proteins (such as...
14 February 2023 969 5 View
I am trying to immunostain whole (micro)tissue samples, and I think there is an antibody penetration issue. Would longer permeabilization procedures or harsher permeabilization agents help with...
14 February 2023 5,892 4 View
Hi, I performed RT-PCR directly on the osteoarthritic cartilages obtained from 2 patients. In both experiments, Ct for TNF-a was found to be around 36, which is unexpected considering the...
31 January 2023 7,269 0 View
I was wondering if it is possible to add a second photoinitiator to SU-8 to photopolymerize it at higher wavelengths. Theoretically, it seems doable, but I'm unsure about the chemistry~
16 January 2023 1,657 2 View
I assume that the antibody recognizes and attaches to the protein from the outer cell membrane. So, maybe there's no need for permeabilization? Bonus question: can we run sequential staining for...
24 November 2022 8,504 2 View
I am trying to characterize the foreign body reaction surrounding my implant, and all the cells are stained positive for CD31. I am not sure if they should be! (I am implanting my material in the...
22 November 2022 8,611 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...
19 July 2024 3,906 0 View