Please help me. I don't know how can I design laparoscope with zemax.
I have fixed human chondrocytes in 6-well plate by glutaraldehyde. For alcian blue staining pH 1, I used this protocol: 1) Rinse wells 3x with PBS. 2) Rinse 1x with 1% acetic acid. 3) Apply...
24 October 2023 7,385 0 View
Any suggestions for high quality miRNA extraction would be appreciated.
13 June 2023 5,958 3 View
I use the typical syringe pumps to load organoids (200-300 um in diameter) into my device; however, I don't see them in the chip! I swapped them with beads (similar size) instead and observed that...
26 February 2023 944 2 View
It's quite confusing to distinguish between the definitions. To me, autofluorescence fits all. But what is the correct terminology to use when talking about biological cells, proteins (such as...
14 February 2023 969 5 View
I am trying to immunostain whole (micro)tissue samples, and I think there is an antibody penetration issue. Would longer permeabilization procedures or harsher permeabilization agents help with...
14 February 2023 5,892 4 View
Hi, I fixed chondrocytes in 12-well plate with glutaraldehyde. I want to stain the cells by direct red. Do you have any protocol? Thank you.
04 February 2023 6,914 0 View
Hi, I performed RT-PCR directly on the osteoarthritic cartilages obtained from 2 patients. In both experiments, Ct for TNF-a was found to be around 36, which is unexpected considering the...
31 January 2023 7,269 0 View
I was wondering if it is possible to add a second photoinitiator to SU-8 to photopolymerize it at higher wavelengths. Theoretically, it seems doable, but I'm unsure about the chemistry~
16 January 2023 1,657 2 View
I assume that the antibody recognizes and attaches to the protein from the outer cell membrane. So, maybe there's no need for permeabilization? Bonus question: can we run sequential staining for...
24 November 2022 8,504 2 View
I am trying to characterize the foreign body reaction surrounding my implant, and all the cells are stained positive for CD31. I am not sure if they should be! (I am implanting my material in the...
22 November 2022 8,611 4 View
can you provide me with a recent case report about BA treated with Laparoscopic KPE ?
11 May 2024 7,951 3 View
I'm a student with an engineering background, and I'm looking to familiarize myself with Zemax and ANSYS for my undergraduate research project, which is my final year project. Could anyone provide...
06 March 2024 4,881 3 View
To build laparoscopic assessment for eye in deep learning give some suggestions.
17 December 2023 2,792 1 View
In the last two decades, one of the main goals of research into laparoscopic intraperitoneal onlay mesh (IPOM) technique for ventral hernia (VH) repair has been to eliminate the transfixation...
05 September 2023 170 0 View
Currently my team are focusing on spectrograph & spectrometer designs using ZEMAX and we can expect that we won't have the license soon. So we are seeking for for the alternative software for...
12 June 2023 7,640 4 View
A parallel beam passing through a 20mm thick pinhole with a aperture of 0.3mm is simulated by using zemax to form a diffractive spot between light and dark on the optical plate.
23 May 2023 9,223 1 View
I tried to design this figure in ZEMAX but I don't how to put the lenses after the ray reflected back.
12 March 2023 756 4 View
I am planning to design a collimatig optical system for a diode laser with a vertical divergence of 15 degrees and a horizontal divergence of 30 degrees. I know that this can be done using a...
17 December 2022 453 10 View
Hello everyone, I need to create VLC V2V communication scenarios on Zemax, however, I am facing several challenges in intercepting the rays with imported CAD objects also the objects seem to...
04 September 2022 616 0 View
I wan to simulate a broad area laser diode on Zemax and I was wondering what value should AI put for the astigmatism. the laser data sheet does not provide a value for the astigmatism but instead...
18 April 2022 1,188 3 View