01 January 1970 1 8K Report

When we study with hypoxia induced exosomes, could CoCl2 be harmful for exosome content in the cell culture media?

More Gizem İlter Aktaş's questions See All
What should we do to solve the clogging problem of Superose 6 Increase 10/300 Column?

We have a Superose 6 Increase 10/300 column (recent photo attached). Recently, we noticed that when we increased to our normal flow rate of 0.5 ml/min, the column reached the pressure limit....

04 June 2024 8,226 0 View

Do you use Artificial Intelligence (such as chatGPT) for research purpose?

The process of formulating research questions and designing methodologies is critical in medical research. As we strive for precision and comprehensive understanding, Artificial Intelligence (AI)...

22 August 2023 1,668 6 View

How many doses of knockdown or overexpression vector were injected into the C57BL/6J mice?

Hello everyone. I'd like to ask some question about C57BL/6J mice experiments. We want to make overexpression and knockdown mice by giving vector. However, we are wondering how many times the...

20 August 2023 3,857 3 View

GC/MS Oven temperature ?

Hello everyone, I just started to use GCMS (8890gc 5977b gcmsd). I want to study human serum metabolomics. But I couldn't get good peaks so far. I keep changing GC and MS parameters but can't get...

20 October 2022 1,962 2 View

Why are the DNA bands not obtained on agarose gel before DNase I treatment ?

I performed RNA isolation from B. subtilis (lysozyme combined with Trizol reagent method) and RNA samples were treated with DNase I. I had samples for running an agorose gel both before and after...

01 September 2022 796 1 View

Is there an online tool for designing hairpin fragments for prokaryotic RNAi-mediated gene silencing applications?

I read an article in which scientists design short and long fragments for a gene which contains an inverted repeat of gene for RNAi-mediated gene silencing in bacteria. But in the article and its...

09 May 2022 1,466 3 View

Is tac promoter compatible with t7 terminator for an expression cassette?

The expression cassette includes a novel gene, two RNAi sequences and overexpression of a gene. I'm planning to place promoter and terminator sequence in between a novel gene, two RNAi sequences...

04 May 2022 6,577 2 View

How to download required packages on R for BRB array tool?

I downloaded BRB array tool, I added the plugin to Excel but I don't have some packages on R so I couldn't analyze. Then I tried to download packages but I see the same packages are missing even...

04 December 2021 3,475 1 View

Arabic NER - Dataset of News & Tweets?

Hello, I have seen two more datasets in Arabic NER evaluation, namely "NEWS" and "Tweets" in the studies in addition to the AQMAR and ANERCORP datasets. However, I could not find the datasets of...

28 September 2021 9,200 3 View

What are the techniques to determine the chromophore/fluorophore conjugated polyclonal antibody quality?

After the conjugation processes, what kind of tests should be used to find the best way of conjugation? How can we know the conjugation reaction efficiency? There is a lot of commercially...

02 March 2021 4,103 3 View

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Could it be a cell culture contamination?

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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Why activated CAR-Jurkat cell could not kill targets?

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How to Add Missing Water Molecules in Protein-Membrane Simulations?

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How Social Media Affects Your Mental Health ?

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Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

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Good Day, can I ask for your help on the questionnaire that I want to adopt?

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