Hello everyone,
I just started to use GCMS (8890gc 5977b gcmsd). I want to study human serum metabolomics. But I couldn't get good peaks so far. I keep changing GC and MS parameters but can't get good peaks. Could somebody help me with the oven temperature? I don't know which temperatures I should use for my method.
According to the different models of the apparatus and the physical and chemical characteristics of the analyte, the temperature program and the type of oven can be changed. Read the following links for getting more information.
https://microbeonline.com/gas-chromatography-gc-principle-parts-and-uses/
https://www.glsciences.eu/optic/gc-injections-manual.pdf
https://alfresco-static-files.s3.amazonaws.com/alfresco_images/pharma/2014/08/22/d436676f-cb9b-4e3e-84d3-45c7ea8153eb/article-18109.pdf
https://www.ecs.umass.edu/eve/facilities/equipment/Agilent6890/The%20Column%20Oven.pdf
Dear Gizem,
you can read this paper about plasma metabolomics by GC-MS:
Molecules 2019, 24, 2367; doi:10.3390/molecules24132367.
If something is not clear, please ask me.
Regards
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