We have a Superose 6 Increase 10/300 column (recent photo attached). Recently, we noticed that when we increased to our normal flow rate of 0.5 ml/min, the column reached the pressure limit. Currently, we can only pass distilled water at a maximum flow rate of 0.03 ml/min, which prevents us from conducting our analyses. Has anyone experienced this issue and found a solution or has any suggestions?

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