I am going to try Oxford Nanopore sequence-specific direct RNA sequencing targeting one of my genes of interest. I have designed the primers and have them with me (Oligo A and Oligo B). In the protocol, it says to combine both the oligos in buffer (10mM Tris-HCl pH7.5, 50mM NaCl). However, there is no mention of getting rid of RNases because I am afraid if I prepare this myself and even autoclave the buffer, it might still have RNases as RNases are probably not inactivated even with autoclaving? Can anyone please suggest if autoclaving is enough to get rid of RNases or there is a better solution to this problem? Thank you very much.