14 Questions 11 Answers 0 Followers
Questions related from Asif Muhammad
Can anyone please suggest some really useful tool to predict RNA secondary structure from a single RNA sequence but it should be capable of predicting structure of RNA at various temperatures....
25 August 2023 2,552 1 View
I am going to try Oxford Nanopore sequence-specific direct RNA sequencing targeting one of my genes of interest. I have designed the primers and have them with me (Oligo A and Oligo B). In the...
12 July 2023 6,232 2 View
I am using Oxford Nanopore RNA sequencing (Direct RNA minION) using total RNA from human cell line but majority of my reads are aligned to mitochondrial genome. Secondly, many reads for the genes...
01 June 2023 622 3 View
I am intending to do minION RNA long-read sequencing using the Direct RNS sequencing protocol. I have successfully been able to quantify the RNA. However, some of the samples seem to have mild...
13 March 2023 4,779 2 View
For the same sample these give different values so which one to trust?
21 February 2023 7,684 3 View
I want to extract good amount of high quality RNA from human lung epithelial cells. I have pellets of nearly 1X10^7 cells per tube. I need very high purity total RNA that can be successfully used...
13 January 2023 1,864 1 View
I have my RNA-seq data analyzed and in my DEG file, there are quite a few MSTRG gene IDs with no gene name like MSTRG.115832.1 (Gene Name: #N/A). DO these correspond to any genes/transcripts or...
10 May 2020 8,103 3 View
I simply wanted to compare differentially expressed genes between 2 types of tissues using RNA-seq. Following, data was processed by a Bioinformatics person and provided 2 types of Excel...
03 May 2020 5,039 2 View
Draining down the sink is always an easy option but not always the right way of disposal. Antibiotics like vancomycin are toxin and are not allowed at any level in animal products for human...
02 May 2017 2,000 3 View
Can individual labs formulate their own procedure for disposal of chemicals or is there a universally recognized system of procedures for disposal of chemicals?
23 February 2016 1,077 2 View
This appeared as contamination in a culture.
03 August 2013 8,428 0 View
The image is of fresh unstained culture under oil immersion. The other image is that of its colonies on LB agar.
12 July 2013 6,606 0 View
I am looking for minimal set of components required for growth of yeast in general.
23 June 2013 10,021 1 View
I was growing my Arcobacter happily using the recipe for Vandamme media (broth and agar). Then suddenly I had either poor growth or not growth in VD agar. All the ingredients were the same except...
15 May 2013 9,262 0 View
