I would like to study Alzheimer but I don't know which cell lines is suitable for it. Can anyone please guide me? Thanks for answers in advance.
Please see the below links:
http://www.nature.com/nature/journal/v515/n7526/full/nature13800.html
http://www.bio-itworld.com/2014/10/13/cell-culture-model-alzheimers-first-recapitulate-key-physiology.html
SH-SY5Y cells are the most commonly used, although the actual cell line you should use depends on what aspect you are going to study.
I did not mean to state that cell-biological studies are not useful but that one should keep in mind that one studies not AD but a minor aspect of the most complex disease of the most complex organ in the universe (the latter holds as long as no aliens have been spotted with better brains than us ...)
Dear Colleagues,
I would like to thank you for taking your time to answer this question.
Respectfully,
Dear Hayder Obayes Hashem,,
I think that I found suitable cell line for my research.
Thanks and Good luck .
I am used all kind of cells, in fact, recently I used oocytes, depend what cellullar aspect you like see.
you can see my paper in alzheimer in my profile
Dear Dr. Fred Van Leuven
I agree with you to say AD is a multifactorial disease, but me as a young student believe that every thing is coming up from genetic bases, even responses to the environment, deciding, enjoying, getting hungry and whatever you think, i believe the life is responses of molecules, all our knowledge, all our thoughts, even our personality or what we think we are, all they are coming up from molecular activities.
Is thinking different from activation and deactivation of some molecular Na+ and K+ pumper proteins of neurons? if so, what we believe as our human being is actually a kind of game of molecules, we are our cells, we are our genes and we are our molecules.
so working on cells to diagnosis a diseases is helpful, but it's important to know which aspect of AD is going to be studied, may be she wants to work on a new chemicals as a new drug, or maybe she wants to study the expression of a unique gene, here we need to work on cells and modeling animals.
Finally i know there is many of many opponents and i thinks this could be my best rejoinder to all objections : Please understand that this is just my personal opinion
best regards
dear Aref,
every "thing" is made of molecules - but "thoughts" are not "things"- precisely THE mystery of human brain: intellect, (self)consciousness, soul, feelings, music, ... must find their origin in more than molecules i.e. in systems operating in our brain that work all or occasionally together too produce a "human with its own mind" - or "minds" because we all have more than one ...
once some of the brain sub-systems begin to fail it is called "trouble" or "disorder" or "disease" or something else - depending on the medical or other discipline
to study such complexness in cells in a plastic cup is both ambitious and naive, so one must tell young people that nerve-cell-biology is a start but far away from "brain"
that was the only point I wanted to make and do not state "I want to study AD in cells", but phrase and define the aim correctly: "to study a molecular mechanism that might be part of the problem in AD"
science is all about "exactness" - also in words !
all the best
emeritus (old) professor Fred Van Leuven
Dear Dr.Leuven
I didn't mean it sir ... as I said it was only my opinion about life ... studying brain is not my field and I was not going to talk about the aspects of brain (and also I have no enough knowledge about it) ... I've just wanted to say that I believe that life is not beyond the world that we know it ... I prefer to believe molecular life instead of supernatural life ...
best regards
As a social researcher I can only guess that there is no known cause or pathway for AD. The longitudinal nun study in USA demonstrated that one person can have plaques and tangles and have AD and another plaques and tangles and not have it. I wonder if it is linked to the gut or liver in some way along with a persons inherited genetics. I think one area of interest to me is the individuality of the inflammatory response to the Plaques and tangles in the brain.
I am sending to your attention updated information on the matter.
Telomere Length Shortening and Alzheimer Disease—A Mendelian Randomization StudyYiqiang Zhan, MD1; Ci Song, PhD1; Robert Karlsson, PhD1; Annika Tillander, PhD1; Chandra A. Reynolds, PhD2; Nancy L. Pedersen, PhD1; Sara Hägg, PhD1
[+-] Author Affiliations 1Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden
2Department of Psychology, University of California, Riverside
JAMA Neurol. 2015;72(10):1202-1203. doi:10.1001/jamaneurol.2015.1513.
Hi,
Currently SHSY5Y but differentiated cells are good model to induce AD like condition using beta amyloid. Rat primary hippocampal culture is another option but tedious.
Justin Conrad Leavitt Would you like to make any comments here. It is an old question but people still posting.
Justin Conrad Leavitt Read the entire log. Someone thinks it's impossible to study AD in intact cells.
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