16 Questions 34 Answers 0 Followers
Questions related from Aref Sobhkhizy
I need to set a protocol to culture the bone marrow cell. I appreciate it if anyone help me.
23 June 2024 7,525 2 View
I've ran a PCR using Hot start Taq polymerase instead of normal taq polymerase by mistake, but as the result i have found some pure and non-sharp bands, may any one help me to find why it has...
05 December 2019 4,116 3 View
I need to evaluate the level of microRNAs in human blood, but I'm not sure which one is more credible, the plasma or serum. I would be very grateful if some one helps me? besides, as you know...
19 April 2016 8,574 3 View
I need to measure the expression of special variant of a gene in mRNA level, but it's not possible to design specific primers for the variant without detecting the other variants. So can anyone...
24 April 2015 7,328 4 View
is it ok to say necrosis will increase the activation and expression of anti-apoptotic genes and pathways? I have some problem in my project which lead me to think that necrosis will increase the...
04 April 2015 7,664 2 View
All we know, centrifuging right after thawing the cells is harmful. But in the other hand, remaining the DMSO in the medium can hit the cells, too. So shall I remove the DMSO or it's better to...
25 December 2014 568 23 View
Is it right to always say the ratio of expression of these 3 variants is similar in different cell types? Which variant has the strongest expression?
23 December 2014 9,000 1 View
My MCF-7 cells are dividing in 5% medium, but I need to keep them alive and prevent them from division...
10 September 2014 7,531 3 View
12 hours after I've passaged my MCF7 cells I found out this unique cell which is disappeared now. Is it fungal contamination or something else?
05 August 2014 2,999 16 View
I need to thaw the frozen MCF7 and T47D cell lines but they are frozen for more than 6 months and I still worry about the storage conditions and percent of alive cells, any tips for the best...
22 July 2014 1,926 36 View
What's the best protocol to isolate the blood plasma in order to save all microRNAs of the fresh blood?
16 April 2014 8,939 2 View
I'm looking for the best performance to extract RNA from some tissues which are stored for more than 1 year or even a couple of years in - 80 degrees of Celsius and we are not sure about its...
08 April 2014 325 6 View
How do miRNA control the methylation? Is there any acceptable theory?
13 March 2014 3,131 2 View
As we know pri-miRNA contains two strands, which one of them is (exactly or partly ) complementary to target mRNA (antisens - miRNA) and the other one is (exactly or partly ) same as a sense...
06 March 2014 988 6 View
I have to deliver two different siRNA at the same time to cancerous cell culture whilst satisfying efficiency.
22 January 2014 3,771 8 View
How TAg and tAg of BK virus induce tumor cells?
06 December 2013 3,256 2 View
