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Questions related from Hayder Obayes Hashim
Hello every one I'm trying to quantitate essential amino acids in serum or DBS by Lc-MSMS(Quadrupole ABsciex 4500 ) . by searching the net for trusted protocol , all protocols that published...
14 October 2021 6,891 1 View
Hello everyone I conducted sequencing prosedure for RNA genome segment (about 700nt) , for this reason I employed the random hexamer primer for cDNA synthesis . after sequencing (by sanger...
17 August 2020 4,169 3 View
Hello researchers I'm trying to dock two ligands simultaneously into the receptor active site to check their interaction to the receptor while they are together . please i want trusted free...
23 June 2020 1,205 2 View
Hello researchers the annealing temperature gradient is the routine step in PCR optimization , but why we do not use annealing time for optimization? regards .
21 August 2017 7,227 14 View
Hello researchers i'm aiming for sequencing 880 nt RNA segment. can i use the ordinary Taq-PCR master mix or i have to use the high fidelity master mix .thank for any suggestions.
19 July 2017 6,616 8 View
I am working on stool samples stored under glycerin , but there is an excess and mixing of glycerin with the stool sample , this lead to lowering the RNA yield due to sample dilution, How can i...
15 March 2017 1,232 1 View
Hello researchersi'm trying to perform a RAPD genotyping for wheat samples . I need any ideas which help me how to start and proceed in optimization of this reaction , and what are the critical...
08 September 2016 4,126 6 View
Hello researchersBoth of coating and low vacuum can be used to observe non conductive samples by SEM , but which one is better for image quality ?I will appreciate any comparisonregards
29 May 2016 6,777 7 View
Hello researchersduring my work on electrophoresis of a certain protein sources , i recognized a good source of positively charged protein .is there any potential application of this proteins ?...
13 May 2016 4,219 3 View
I extracted DNA from several hundreds blood sample by promega kit , one sample among all the others show a two clear fluorescent bands migrate to the negative pole , those bands appear without any...
27 January 2015 4,000 13 View
Hello every one I'm thinking to purchase DNA oligo synthesizer for small commercial purpose , and indeed I have no idea about it . any help will be a appreciated , especially about 1- The good...
12 January 2015 1,356 9 View
In deed im thinking about conducting a haplotype project to a certain population ( other than the populations that have been mapped by other project ) The question is : is the haplotyping of a...
27 December 2014 2,452 1 View
Maybe we can read anywhere that the human chromosomes were numbered according to their size , i.e. chromosome 1 is bigger than chromosome 2 and chromosome 2 is bigger than chromosome 3 and so on...
12 December 2014 5,400 4 View
I am studying the association of certain SNP with a specific disease, and due to several reasons I will use PCR-RFLP method for SNP genotyping, now I have two options:a- To design and optimize my...
27 November 2014 3,820 3 View
