Hayder Obayes Hashim

9 Questions 33 Answers 0 Followers

Questions related from Hayder Obayes Hashim

Serum or DBS amino acids analysis without labeled internal standards ?

Hello every one I'm trying to quantitate essential amino acids in serum or DBS by Lc-MSMS(Quadrupole ABsciex 4500 ) . by searching the net for trusted protocol , all protocols that published...

14 October 2021 6,876 1 View

Can oligo hexamer primer produce sequencing error?

Hello everyone I conducted sequencing prosedure for RNA genome segment (about 700nt) , for this reason I employed the random hexamer primer for cDNA synthesis . after sequencing (by sanger...

17 August 2020 4,129 3 View

Any free software for Simultaneous Docking of Multiple Ligands?

Hello researchers I'm trying to dock two ligands simultaneously into the receptor active site to check their interaction to the receptor while they are together . please i want trusted free...

23 June 2020 1,188 2 View

Annealing time or annealing temperature which one is better for PCR optimization ? and why?

Hello researchers the annealing temperature gradient is the routine step in PCR optimization , but why we do not use annealing time for optimization? regards .

21 August 2017 7,203 14 View

Is it essential to use high fidelity-pcr prior to sequencing ?

Hello researchers i'm aiming for sequencing 880 nt RNA segment. can i use the ordinary Taq-PCR master mix or i have to use the high fidelity master mix .thank for any suggestions.

19 July 2017 6,597 8 View

How to recover rotavirus from samples stored under glycerin?

I am working on stool samples stored under glycerin , but there is an excess and mixing of glycerin with the stool sample , this lead to lowering the RNA yield due to sample dilution, How can i...

15 March 2017 1,215 1 View

Which one is better for SEM image coating or low vacuum?

Hello researchersBoth of coating and low vacuum can be used to observe non conductive samples by SEM , but which one is better for image quality ?I will appreciate any comparisonregards

29 May 2016 6,764 7 View

Is there any industrial or medical application for positively charged protein?

Hello researchersduring my work on electrophoresis of a certain protein sources , i recognized a good source of positively charged protein .is there any potential application of this proteins ?...

13 May 2016 4,207 3 View

What is this fluorescence material in my DNA sample ?

I extracted DNA from several hundreds blood sample by promega kit , one sample among all the others show a two clear fluorescent bands migrate to the negative pole , those bands appear without any...

27 January 2015 3,984 13 View