Replacement for erythrocytes (RBCs) for carrying out lectin - hemagglutination assays?

For an experiment I have to test for presence of lectins in a plant extract. I shall be carrying out the regular hemagglutination assay. However, I wish to avoid the use of erythrocytes. So I want...

03 April 2019 2,854 1 View

Is there a protocol for immortalization of corneal epithelial cells?

I am working on inflammatory responses of corneal epithelial cells to certain stimuli. We have procured primary corneal cells for this purpose. However, the primary cells can be passaged for 4-6...

31 December 2013 4,292 1 View

Is there a "native protein" induction system available for fungi?

I am working on fungal lectins. I am required to purify a cytoplasmic lectin from a opportunistic fungal pathogen species. The yield has been very low in the past few months. I want to know if...

11 December 2013 4,888 3 View

Lectin production in fungal mycelia over time?

I am purifying a lectin from a certain fungal mycelia. In brief, I am growing Acremonium in Brydes liquid media containing dextrose as sole carbon source and ammonium nitrate is the only nitrogen...

08 September 2013 8,410 2 View

Immobilization of proteins on PVDF membrane

This is regarding the ability of PVDF membrane to immobilize proteins onto it. If I run the transfer for prolonged periods, will the proteins stick onto the membrane or do they pass through the...

07 August 2013 8,341 7 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View