Suhas Ballal

6 Questions 5 Answers 0 Followers

Questions related from Suhas Ballal

Replacement for erythrocytes (RBCs) for carrying out lectin - hemagglutination assays?

For an experiment I have to test for presence of lectins in a plant extract. I shall be carrying out the regular hemagglutination assay. However, I wish to avoid the use of erythrocytes. So I want...

04 April 2019 2,909 1 View

Is anyone familiar with Methanol/Ethanol precipitation of a desired protein fraction from a small volume sample, such as 10 ml?

I am in the process of concentrating a lectin from the crude extract of fungal mycelial mat. I want to approach this problem by organic solvent precipitation by sequential increase in %...

10 October 2014 3,475 2 View

Is there a protocol for immortalization of corneal epithelial cells?

I am working on inflammatory responses of corneal epithelial cells to certain stimuli. We have procured primary corneal cells for this purpose. However, the primary cells can be passaged for 4-6...

01 January 2014 4,329 1 View

Is there a "native protein" induction system available for fungi?

I am working on fungal lectins. I am required to purify a cytoplasmic lectin from a opportunistic fungal pathogen species. The yield has been very low in the past few months. I want to know if...

12 December 2013 4,921 3 View

Lectin production in fungal mycelia over time?

I am purifying a lectin from a certain fungal mycelia. In brief, I am growing Acremonium in Brydes liquid media containing dextrose as sole carbon source and ammonium nitrate is the only nitrogen...

09 September 2013 8,444 2 View

Immobilization of proteins on PVDF membrane

This is regarding the ability of PVDF membrane to immobilize proteins onto it. If I run the transfer for prolonged periods, will the proteins stick onto the membrane or do they pass through the...

08 August 2013 8,374 7 View