How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

How do you obtain an even spread of polymer film on Silicon wafer?

Hi Everyone, I made some polymer(Polylactide) films on Silicon wafer. The polymer was dissolved in DCM and THF. I observed that the colour of the film at the centre of the Wafer is different...

27 January 2021 9,724 1 View

What could cause a loss of detector response for a brief period of time in UHPLC/MS?

The following have already been done, 1. Solvent change and fresh new samples were prepared.

03 December 2020 8,532 1 View

What utility function would be used to reward/punish learning of non local rules defining implicate order?

Given the Meijer paper "A New Premise for Quantum Physics, Consciousness and Fabric of Reality," I am developing an OpEMCSS systems science simulation model. The system is a network of concurrent...

23 November 2020 6,493 1 View

Professional Engineer's Code of Ethics: What & How To Assure Compliance?

Historically, the subject question has been met mainly with "After The Fact" assertions. Yet when one learns of the infrastructure failures . . .bridges, tunnels, pipelines, highways. . . it...

11 November 2020 3,574 3 View

There is a GLMM for proportion structure data?

I would like to ask a recommendation of a statistical test to answer a question related to an unusual data structure. I am testing if birds change nest parental care behavior depending on...

17 October 2020 2,026 1 View

Hello everyone, good day! In the process of deproteinization of samples to obtain chitin, can I make use of a phenol as a deproteinizing agent?

I would like to know what other reagents can be used to obtain chitin by deproteinizing a sample. Commonly found on articles is the employment of NaOH concentrations as well as KOH. I would like...

16 October 2020 537 6 View

What do you think are the factors affecting the Online Collaborative Approach of the teachers in today's situation?

This is research-related question.

05 October 2020 4,606 4 View

What emotional safety protocols can be put in place as "first aids" to deal with our anxieties and fears owing to Covid-19?

A lot of physical safety protocols have been upheld all around the globe since the Covid-19 pandemic. What i do not hear often is how to deal with the emotional and psychological distress that...

26 August 2020 1,516 32 View

Q1. What changes have been observed in the professional lives of our CE graduates ? Q2. What CE academic programs have developed?

In June 1995, the culmination of some 3 years or so of preparation, led by professor Dr. Jim Yao, Texas A&M, resulted in an "ASCE Education Conference" in Denver, CO. At the end of that...

12 August 2020 1,069 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

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28 February 2021 9,936 2 View