Does Anyone Know of a Way to Detect Melatonin that is NOT HPLC, RIA or Mass Spec?

I tried to do a Slot Blot and was not successful and melatonin is only .23 Kda so it would probably run off a western blot. Has anyone had success with other methods?

23 January 2021 1,388 3 View

Would anyone be able to provide me with the book with the title Globalization: A Very Short Introduction?

In view of the research question I posted recently, this book will be of great importance, however, I can't get the soft copy so I was hoping if anyone happens to have it and would really...

24 December 2020 7,199 3 View

Any advice or articles on combining one qualitative approach with the methods of a different approach?

I am a doctoral student and I am looking to do a qualitative research study that looks at the potential influence of teachers on the way minority students view their leadership capabilities....

28 November 2020 6,217 5 View

Troubles cloning - correct RE digestion and then incorrect sequencing?

I am inserting an MPP5 gene insert into a pCAGGs backbone. When I am finished with the mini prep, I find that my RE digestion is correct, but then when I send it to be sequenced, the MPP5 gene is...

23 November 2020 7,962 2 View

Hello researchers, can anyone help me with this Structural Decomposition Analysis (SDA)?

I am using Economic Input-Output Life Cycle Assessment (EIO-LCA) model I need to decompose this equation W= P*F* L* Yc* Yg to generate 120 forms. where W is a vector of total water withdrawal...

20 November 2020 4,194 3 View

How has Globalization affected development in the Global South?

I am a student working on a long essay with my topic as "Globalization; an additional problem or a solution to the problems of the global South countries". In this paper, I seek to discuss the...

26 October 2020 3,966 4 View

How to convert a cif file to a qe file?

I am trying to convert a cif file to a Quantum espresso file to do DFT calculations

10 September 2020 1,535 4 View

What are the available Nanocomposite Databases?

I am looking for data files of Nano Composites. Any form of data, be it XRD outputs, SEM or TEM images or anything. Where can I find them?

28 August 2020 9,927 5 View

Can I degas drug solutions before applying to mammalian cells for electrophysiology?

I am applying different drug solutions to cells that I am whole-cell patching. Each solution is in a syringe connected to a thin tube that goes through a barrel manifold into a glass micropipette...

24 August 2020 1,956 2 View

How to count % GFP positive cells and GFP intensity using imagej?

I have infected BHK21 cells with a virus that expresses GFP. I want to count the percentage of GFP positive cells and the intensity of the GFP. How do I do this with fluorescent microscope...

17 August 2020 4,009 2 View

Coronal or Sagittal mouse brain sections to visualise vessels?

Hello, I am a first year PhD student and I am working on Cerebral amyloid angiopathy (CAA). I would like to investigate the presence of my proteins of interest in a CAA/Alzheimer's disease mouse...

05 December 2020 907 3 View

Has anyone managed to install Abaqus 2019 on manjaro linux?

I'm trying to install abaqus on manjaro Linux but have been unable to do so. In the Linux.sh file I commented out the DSY_OS_Release=`lsb_release --short --id |sed 's/ //g' line and replaced it...

09 October 2019 476 2 View

My drugs have higher CAA values than quercetin. Is this logical or did I do something wrong?

Hello, I performed CAA assay with N-acetylcysteine (both free and nano-formulated drugs), quercetin on SHSY-5Y cell line. However, CAA values for those drugs are higher then the quercetin. Any...

07 August 2019 5,475 1 View

Does anyone have experience using the Chat-Cre mice lines from Jackson? I am having trouble getting the PCR bands to show up during genotyping?

'GCA AAG AGA CCT CAT CTG TGG A' - WT Forward Primer 'CAG GGT TAG TAG GGG CTG AC' - Common Primer 'CAA AAG CGC TCT GAA GTT CCT' - Mt Forward Primer PCR Protocol is attached. In brief, used as low...

02 March 2019 6,902 6 View

Has anyone done PCR for Tac-1 mice (Jax, stock:021877 )?

11 December 2018 4,893 4 View

2019 CFD/CAA conferences/workshops ?

Hi everyone, Does anyone know about high level CFD/CAA conferences/workshops taking place next year in 2019-2020? Any suggestions will be much appreciated. Thanks a lot.

10 November 2018 196 1 View

What are the possible number of mutation in a single Codon?

I work on Bioinformatics system for mutation detection in TP53, I would like to know the possible number of mutation per a codon i.e.: Codon 248 consist of three bases of amino acid which...

07 August 2018 511 5 View

Which cancer cell line is recommended for cellular antioxidant activity study using plant extracts?

We have HCT116 and HepG2 cancer cell line in our lab, in which we are planning to use either one of them. The research article below suggests to use Caco-2 cell line instead of HepG2. I would like...

06 March 2018 359 7 View

Which cancer cell line is recommended for cellular antioxidant activity study using plant extracts?

We have HCT116 and HepG2 cancer cell line in our lab, in which we are planning to use either one of them. The research article below suggests to use Caco-2 cell line instead of HepG2. I would like...

02 March 2018 9,713 8 View

Can anyone suggest me how Islamic banking or financial organizations uses social media networking tools?

07 August 2016 6,064 0 View