in slides under microscope, only parts of the tissue or other things are found and the comet tail is not found.
Milad in my opinion the lysis of nucleous is not right. When lysis is weak DNA inside nucleous is intact and it could be a reason why you can not see the comet tail. You should see also the electric field in your agarose gel. The agarose should be low point fussion.
luis
thanks luis, i making lysis buffer freshly and daily. im sure that lysis buffer is Ok.
Have you tested your reagents? This is a histological stain adapted from Tollens test. Unfortunately the reagent degrades quite easily and should be stored carefully in low temperatures out of direct light. Should be able to do a silver mirror precipitation test in a test tube to check
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