My aim is to test anticancer properties of medicinal plant and its endophytic fungi extract in methanol.using human-derived liver cell line.Our target groups are polyphenols and flavonoids.
So methanol can be used as a solvent for dissolving the extracts in the cell lines?,if not, What is/are the best solvent(s) should be used ?, and what is the maximum concentration of the solvent that can be used for dissolving?
Thank you very much!
I think !ethanol could not be used to dissolve extracts in anticancer assays since it has a cytotoxic acivity.
I assume that the question is "may I dissolve my extract in methanol and give to ym cell line?"
The answer is no- however, DMSO may be used to dissolve extracts followed by dilution with water. 10% is the maximum I've heard being used for assays, but your cells may not tolerate that concentration- you''ll need to test that.
I have not recommended the use of methanol to dissolve extracts or other compounds, because of their interference in the cytotoxicity of compounds. Unless the test is performed demonstrating negative control with methanol concentrations used to dissolve the samples.
To solve this we have always used DMSO, which has been a good solvent for use in cytotoxic tests. The maximum concentration of this should be 0.5%.
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