15 February 2020 2 653 Report

Cells were shipped on ice from a collaborator in NY to California, then were immediately stored in liquid N2 until the day of thawing. Cells were diluted in MEF media (DMEM, FBS, penicillin strep), spun down at 300xg for 5 mins and resuspended in hESC media and plated on MEFs. I am preparing to thaw another vial, any tips?

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