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18 November 2020 9,422 1 View

What is the good method for cleaning tungsten fiber before coating process?

I am working on tungsten fiber coating. My first step is to remove oxide contaminants on the surface. Please suggest me some Pre-treatment methods.

19 May 2019 5,741 3 View

How to calculate residual stresses for graphene using Raman sprectroscopy ?

After obtaining Raman spectroscopy of Graphene before and after composite sintering. The residual stresses in graphene to be calculated.

22 June 2018 1,863 3 View

Can I know how to do sensitivity analysis using R for the the given data??

The 2 columns variables and values are the input to some design and I observed those remaining column values as output. Please tell me how to write R program to have sensitivity analysis for such...

08 November 2017 9,141 1 View

During Spray Deposition process, if a billet/substrate experiences stress higher than yield, does it mean that substrate material failed(not usable)?

Or is it okay for the substrate to experience such high thermal stress?

27 August 2017 6,892 2 View

How to solve non-positive volume cells mesh issue in icepak?

Exact message "Warning: The mesh contains non-positive volume cells.This must be fixed before solve." The model is made up of .igs CAD file import. It has curved surfaces. Is there a way to check...

30 July 2017 237 3 View

HI, I need help to analyze the data obtained by in vivo recording of spontaneous activity from locus ceruleaus of mouse brain.

I would like to know in detail (step by step) how to analyze the data for spike detection and sorting using Axograph. Please find the raw data as attached. Thank you very much ! Please do to...

30 July 2017 6,075 1 View

How to identifying & charecterization of lactobacillus & Bifidobacterium? ??

25 July 2017 9,836 2 View

Bifidobacterium ::: how to isolate the colonies from capsules ?

17 July 2017 3,536 3 View

Is there any book recommeded for spectrum sensing methods?

06 May 2017 4,110 4 View

How to Preserve Murine splenocytes?

Dear Researchers, I would like to know if there is a method to preserve murine splenocytes without staining for future FACS analysis for immunophenotyping? Thanks in Advance. Mohd Najib Mostafa

02 March 2020 403 2 View

Any suggestions for Monocyte population disappeared after surface staining from Flow Cytometry?

Hi guys, I am doing surface staining for monocytes, macrophages ( CD14, CD16), and lymphocytes using thawed PBMCs. My lymphocytes looked perfectly fine but monocytes were all disappeared (or...

05 June 2019 9,902 10 View

Only CD8 positive cells in hPMBC humanized model, no CD4 cells?

We have been using hPBMC-NOG mice for humanized mice. After tumor has grown to a 800mm3, we have done immunophenotyping of these tumors. We consistently observing that CD4+ cells are completely...

04 April 2019 8,449 1 View

Only CD8 positive cells in hPMBC humanized model, no CD4 cells?

We have been using hPBMC-NOG mice for humanized mice. After tumor has grown to a 800mm3, we have done immunophenotyping of these tumors. We consistently observing that CD4+ cells are completely...

03 April 2019 9,616 1 View

Can anyone suggest good free flow cytometry software that can read the ncf extension files?

Hi all, i currently do immunophenotyping experiment using novocyte express machine. Im looking for free software for analysing the data including setting the gate, compensation etc etc. The...

08 January 2019 1,290 5 View

Can anyone suggest good free flow cytometry software that can read the ncf extension files?

Hi all, i currently do immunophenotyping experiment using novocyte express machine. Im looking for free software for analysing the data including setting the gate, compensation etc etc. The...

31 December 2018 1,879 5 View

Can anyone explain whether a Flow cytometry and IHC data comparable?If yes then what parameters to be considered?

I am working on Flow cytometry immunophenotyping with tissue samples.In Flow we generally consider the parent percentages of our gated population of interest.But how to correlate it with...

17 December 2018 1,492 2 View

What is the best recipe for preparing tissue lysis buffer?

I am looking for a tissue lysis buffer for immunophenotyping. TQ

11 December 2018 8,084 0 View

Can anyone explain whether a Flow cytometry and IHC data comparable?If yes then what parameters to be considered?

I am working on Flow cytometry immunophenotyping with tissue samples.In Flow we generally consider the parent percentages of our gated population of interest.But how to correlate it with...

11 December 2018 8,760 2 View

I could not find any specific work on PP242 and Tuberous Sclerosis and / or lymphangioleiomyomatosis. Can someone tell me something about ?

PP242 Counteracts Glioblastoma Cell Proliferation, Migration... mTORC1 and mTORC2 in cancer and the tumor microenvironment...

05 June 2018 7,254 3 View