What pH buffer solution best replicates physiological conditions?

I am studying the interaction between protein (HSA) and liposomes (made with DMPC) and I would like to study under physiological pH for application in studying protein interaction with DDS. I have...

10 November 2020 3,594 3 View

All teacher educators are under the lockdown since March, what could they be doing, how is this lockdown going to affect tutors' performance ?

There are many COVID 19 related issues in terms of how to deal with the continuum of teaching and learning. I am meditating about what the future performance results will be for both the student...

16 June 2020 199 3 View

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03 December 2019 5,253 1 View

Reliable conjugated and unconjugated IL17-F anti-human antibody for flow cytometry?

Dear all, can someone kindly recommend a reliable fluorochrome-conjugated IL17-F anti-human antibody and also an unconjugated one please? I am studying CD3+ Lymphocytes from healthy volunteer...

19 February 2019 3,843 3 View

What is an acceptable sampling rate for skin conductance?

What is an acceptable sampling rate for measuring skin conductance? Research seems to use various rates, from 32Hz to 1000Hz. Is there a minimum rate advised? I have a device that measures at 4Hz...

03 February 2019 9,630 1 View

Is it possible to prepare SDS Reducing Buffer without including the bromophenol blue?

Hi all, I am doing an SDS-PAGE (Laemmli) gel electrophoresis but don't have any bromophenol blue to make up my SDS sample buffer. Is it possible to leave this out if you are confident of how...

26 January 2018 2,096 3 View

How to calculate the Feed Conversion Rate (FCR) in free-choice diets?

Hi! I would like to calculate the FCR for a diet-induced obesity experiment in rats. Since I have many nutrients/groups, I am calculating the food intake (FI) in kcal, not in grams of food. Has...

09 November 2017 5,258 5 View

What is the typical percentage error when corneal endothelial cells are counted using a specular microscopy ?

11 July 2016 7,906 2 View

How do I design Methylight primer/probes without using Beacon?

I want to Methylight assays and I need to be able to design the primer/probe sets, or get software that can do it for me. But I've only been able to find Beacon software that does both primers and...

08 June 2016 9,414 1 View

What Australian cities would be good to compare with Geelong, Australia in regard to economic, social & physical capital?

Newcastle and Wollongong are good examples but I am interested to learn about other large regional centres, particularly those that may have experienced a shift in their economy and have had to...

26 November 2015 5,759 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

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03 March 2021 5,360 2 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View