Anti-Iba1 antibody for westernblotting?

I am planning to do a westernblot for the microglia marker Iba-1, using mouse brain extract . Please recommend a good antibody for westernblotting.

17 October 2018 1,090 5 View

How to calculate residual stresses for graphene using Raman sprectroscopy ?

After obtaining Raman spectroscopy of Graphene before and after composite sintering. The residual stresses in graphene to be calculated.

22 June 2018 1,863 3 View

How to plot a graph for TF*IDF values output in pycharm?

i already have output for TF*IDF output inpycharm...i required to plot a graph term and TF*IDF and document similarity and qurery comparision with documents. please suggest me using python code in...

29 May 2018 3,375 4 View

Can anybody help me to design transmission over time varying channel in MATLAB?

I am writing code for multi-carrier data transmission over time varying multi-path channel in MATLAB. Mathematically , received signal can be represented as, y=x(t)*h(t,delay)+n(t) Impulse...

20 May 2018 943 2 View

Is there any alternative of acetic anhydride for the preparation of perchloric acid?

i have to measure protein concentration of bacteria on polyethylene surface. for this i used coomassie brilliant blue solution in perchloric acid as an indicator. but acetic anhydride is not...

18 February 2018 6,826 2 View

Can anyone suggest under what conditions FWHM (In Raman studies) will decrease with incresing conc. of aromatic molcules -TCNE with Few layer MoS2?

I have done in detail Raman studies for charge transfer between few layer MoS2 and donor and acceptor aromatic molcules (like TCNE, MV etc.).In general FWHM increase with increasing conc. of...

23 August 2017 9,529 2 View

HI, I need help to analyze the data obtained by in vivo recording of spontaneous activity from locus ceruleaus of mouse brain.

I would like to know in detail (step by step) how to analyze the data for spike detection and sorting using Axograph. Please find the raw data as attached. Thank you very much ! Please do to...

30 July 2017 6,075 1 View

How to identifying & charecterization of lactobacillus & Bifidobacterium? ??

25 July 2017 9,836 2 View

Bifidobacterium ::: how to isolate the colonies from capsules ?

17 July 2017 3,536 3 View

Can any one suggest suitable reference or procedure to find presence of elements or compounds from XRD peaks in Xpert high score plus software ?

Sample is ZrB2 (Zirconium Diboride )powder.

01 May 2017 8,424 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View