I am immunophenotyping PBMCs by flow cytometry using markers for T cells to analyse the different subsets (Naive, CM, EM and EMRA), and to measure senescent and exhausted t cells. I want to evaluate the effects of 2 different interventions on these cells, so in order to do the statistical analysis to compare pre and post intervention values and between groups values, what parameter should I use? frequencies of each population? MFI?
Thank you!
James Leigh
Not sure if you can get MFI for subgroups but if so you could do a one way ANOVA of post - pre MFI across groups.
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