Immortalised BALB/c derived, dendritic cell line?

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Does anyone have information on the tolerance in different animals after i.v. administration of nitisinone?

I am interested in the safety/toxicity of NTBC administration in different animals. There is a lot of data available relating to oral administration however limited data on intravenous administration.

04 June 2024 5,628 1 View

Does the Sysmex DI-60 detect any malarial parasite in the blood? How accurate and precise can this automated machine detect malarial parasites?

According to the journal article I've posted, the Sysmex DI-60 can detect the different morphologies of the RBCs. I want to find out if the Sysmex DI-60 can detect not only the morphology of the...

18 March 2024 8,225 3 View

Why is K2 EDTA blood test tube more recommended to use in blood collection than K3 EDTA blood test tube?

The journal that was given to us stated that they recommended to use K2 EDTA blood test tubes to collect the blood samples of the patients.

06 February 2024 3,607 2 View

What are the usual limitations of blood gas analysis?

I am in need of answers as to knowing the limitations of blood gas analysis for our report.

07 August 2022 5,558 6 View

Inducing electrostatic conductivity on polymers with high surface resistivity?

I am wondering if there are recommendations on how one can create electrically conductive surfaces on polymers such as PLA, PCL, PLGA? Thanks in advance.

27 August 2019 5,043 3 View

Ways to generate single micro/nanofibers?

I am looking for suggestions on how to generate individual fibers (either monofilament/yarn/bundles). I have used melt spinning, wet spinning, and yarn spinning to produce fibers with diameters...

12 June 2019 7,902 6 View

How to configure BAL bioreactor with oxygenator?

I am wondering if anyone has a simple blueprint on setting up a BAL bioreactor? Specifically, we have engineered the machine except we are wondering about the oxygenator - will atmospheric oxygen...

28 May 2019 4,868 2 View

Efficient way to cut fibers at 1 mm or below?

I am wondering what kinds of techniques are used to cut fiber segments to lengths of 1 mm or less (common in flocking industry). Currently, cutting is done by hand and is too tedious to be...

23 April 2019 1,032 3 View

Bacterial Overgrowth on NGM Plate?

There has been a recurrent problem in our NGM plates - our OP50 continues to grow over time and travel with the worms. Does anybody else have this problem - and how did you fix it? We switched to...

03 February 2019 4,029 0 View

Why does my perovskite solar cells always suffer from low Jsc and FF?

Recently I step into this field as a rookie, my cell is FTO/bl-TiO2/mp-TiO2/MA0.17FA0.83PbI2.49Br0.51/X60+LiTFSI+t-BP/Au. Blocking layer from ALD system has thickness ~50 nm, mp-TiO2 is...

08 January 2019 7,155 7 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,105 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 640 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,892 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View