What is the most appropriate emsemble to use for simple AA membrane simulations?

I am working on some more complex membrane simulations and wanted to know if there is a specific advantage to using a certain ensemble. It seems as though most people have used either NVE, NVT, or...

20 November 2019 1,307 3 View

Efficient way to cut fibers at 1 mm or below?

I am wondering what kinds of techniques are used to cut fiber segments to lengths of 1 mm or less (common in flocking industry). Currently, cutting is done by hand and is too tedious to be...

23 April 2019 916 3 View

How can I evenly spread a viscous ANF dispersion between two glass plates without forming air bubbles?

I am currently working on creating Aramid Nanofiber thin films for my research project. What we do currently is put a large dollop of the ANF on a glass plate that is in a spin coater. We spin it...

15 March 2018 9,461 3 View

How do I check for statistical significance between 3 groups using percentage values determined from categorical data?

Hello, thanks in advance for help with what I suspect is a simple query but it's beating me. E.g. from the cohort (3 groups) x%, y% & z% took the tube to work, is there a statistical...

25 September 2016 3,397 13 View

Any particular reason(s) for using SiHa, HCT116, HT-29, and FaDu cells in tumor hypoxia studies?

SiHa, HCT116, HT-29, and FaDu cell lines have been widely used in tumour hypoxia studies. Any particular reason(s) for selecting those lines? Thanks everyone :)

01 June 2016 6,199 2 View

Any experience using an absorbance reader to assess cell titer blue assay?

Does anyone have experience in using absorbance readers (570nm/600nm) in cell titer blue assay (as opposed to the recommended fluorescence)? I am planning on using the Biotek ELX800 for this.

01 May 2016 6,071 3 View

Is there a common neuroectodermal origin of human platelets and spinal dorsal horn?

I know I read this in a paper detailing the rationale for work in the 1980s where platelet responses were used as model to study the effect of antidepressants and antipsychotics prior to more...

28 August 2015 2,446 5 View

What is the setup for a 3 electrode EIS using a Solartron 1260 in conjunction with a 1287? And how can this setup be verified with ZPlot?

I have been setting up an EIS system using a Solartron 1260 in conjunction with the 1287 model and a Princeton Research Flat 3 electrode cell. From my understanding, the 1287 is hooked up to the...

23 March 2015 1,832 3 View

Any tips on how to carry out a fluorescent LDL uptake study?

Am currently working on patients with various genetic cholesterol defects. We take skin biopsies and culture fibrobalsts for DNA and for in vitro testing. To confirm the genetic finding we are...

24 July 2014 1,597 2 View

Chlorpromazine Hydrochloride on U87MG cells

I am looking for advice. I'm currently using Chlorpromazine Hydrochloride on my U87 cells at various concentrations (2.5ug/ml, 5ug/ml and 10ug/ml), however all concentrations are causing...

01 January 1970 684 1 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Which is the most convenient manufacturing software for Additive Manufacturing?

Which tools can be used to generate machine codes for additive manufacturing?

02 March 2021 7,220 2 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

How can we add reinforced thermoplastics with granules in injection molding machine?

I need to make an injection molding machine in which short fibers are to be introduced. Short fiber length and material properties are to be determined and also the material strength are to be...

22 February 2021 4,290 1 View

What are some underexplored theories and concepts in Activated Carbon as A Water Filter?

Hi, me and my team mates are currently planning to pursue the topic of Activated Carbon from Coconut as a Water Filter. And we would like to ask for your suggestions and knowledge on what specific...

22 February 2021 6,569 3 View

Why is my qPCR suddently not working?

I've been working with qPCR for some months now. I amplify 2 genes (PSEN2 and HN1), and the protocols are optimized for both and have been working fine. The other day I amplified HN1 for some...

21 February 2021 3,437 3 View

What are some underexplored theories and concepts in Activated Carbon as A Water Filter?

Hi, me and my team mates are currently planning to pursue the topic of Activated Carbon from Coconut as a Water Filter. And we would like to ask for your suggestions and knowledge on what specific...

21 February 2021 7,561 4 View