Can MeDIP-seq (methylated DNA immpunoprecipitation sequencing) be performed on DNA previously stained with DAPI or Hoechst?

Hi All, Can MeDIP-seq (methylated DNA immunoprecipitation-sequencing) be performed on DNA previously stained with DAPI or Hoechst? If not, are there any DNA stains compatible with the...

13 November 2016 2,487 1 View

What is the function of sucrose in lysis buffer for isolation of intact nuclei?

What is the function of sucrose in lysis buffers for isolation of intact nuclei? I observed that nuclei pellets resuspend easier in buffers without sucrose, and have a higher tendency to clump...

14 June 2016 8,320 10 View

What is the best strategy for FACS staining of nuclear protein for sorting fixed nuclei?

Hi All, I need to FACS-sort formaldehyde-fixed nuclei from mouse brain tissue. What is the best strategy for staining of intranuclear protein for immunolabeling for FACS (staining of soluble...

14 May 2016 5,025 3 View

Has anyone came across a decent IL-8 murine ELISA or bead assay?

I'm looking for an IL-8 mouse specific ELISA but can't find any (at least via mighty google). I'd appreciate any suggestions. Lech

08 January 2015 798 4 View

Are YBCO or similar high temperature superconductors already applied in industry?

I can remember very well the discovery of superconduction effect in ceramic materials in the eighties and I am curious where and how this discovery has finished. Run any research projects in the area?

19 May 2013 5,712 4 View

Do you know the industrial application of the photocatalytic effect on titania surfaces?

The effect was shown by Fujishima and Honda in 1972 and occurs in the presence of titania, water and uv radiation. It leads to the degradation of pollutants and may be for example applied to the...

19 December 2012 6,407 12 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

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02 March 2021 3,060 3 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View