Is it okay for biopsy samples to be stored for about 24 hours in CELLBANKER Solution at 4 degrees Celsius?

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ReproSil NH2 column for sugars analysis - mobile phase?

I have five different columns from Dr.Maish: 1) ReproSil - XR 120 NH2 2) Reprosil 80 NH2 3) Reprospher 100 CN 4) Reprosil - Pur 120 NH2 5) Reprosil Saphir 100 NH2 4) Reprosil - Pur 120 NH2 Can I...

08 June 2022 2,467 0 View

What are the possible ways to improve recombination efficacy of Cre- and Flp- dependent STOP cassetes?

Cre recombination efficiency often impacts activation of Cre/LoxP dependent transgenes. We have recently encountered problems with recombination in certain cell types in vivo, especially with...

11 August 2019 7,555 3 View

Can MeDIP-seq (methylated DNA immpunoprecipitation sequencing) be performed on DNA previously stained with DAPI or Hoechst?

Hi All, Can MeDIP-seq (methylated DNA immunoprecipitation-sequencing) be performed on DNA previously stained with DAPI or Hoechst? If not, are there any DNA stains compatible with the...

13 November 2016 2,637 1 View

What is the function of sucrose in lysis buffer for isolation of intact nuclei?

What is the function of sucrose in lysis buffers for isolation of intact nuclei? I observed that nuclei pellets resuspend easier in buffers without sucrose, and have a higher tendency to clump...

14 June 2016 8,558 10 View

I'm looking for a good antibody against mouse nuclei, suitable for FACS - any recommendations?

Hi All, I'm looking for an antibody directed against mouse nuclei (not cell type-specific), preferably directed to component(s) of nuclear envelope, and being a monoclonal. Antibody is intended...

06 June 2016 4,184 1 View

What is the best strategy for FACS staining of nuclear protein for sorting fixed nuclei?

Hi All, I need to FACS-sort formaldehyde-fixed nuclei from mouse brain tissue. What is the best strategy for staining of intranuclear protein for immunolabeling for FACS (staining of soluble...

14 May 2016 5,244 3 View

Is it possible to use Miltenyi MACS micro Beads and/or uMACS beads in tubes instead of columns?

Hi All, I was just wondering if anyone tried to use any of the Miltenyi beads in tubes instead of columns, with a standard magnets compatible with the "tube" magnetic separation format. My guess...

03 May 2016 304 3 View

Can Taq polymerase be used in SOE PCR?

Should using Taq polymerase be avoided when when doing SOE PCR? I would assume that, due to addition of non-template A and lack of exonuclease activity, it would generate mutant extension...

18 July 2015 4,137 5 View

Has anyone came across a decent IL-8 murine ELISA or bead assay?

I'm looking for an IL-8 mouse specific ELISA but can't find any (at least via mighty google). I'd appreciate any suggestions. Lech

08 January 2015 943 4 View

Are YBCO or similar high temperature superconductors already applied in industry?

I can remember very well the discovery of superconduction effect in ceramic materials in the eighties and I am curious where and how this discovery has finished. Run any research projects in the area?

19 May 2013 5,832 4 View

Could you recommend some articles on Urban Transportation System optimization and Innovation?

13 August 2024 2,595 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?

02 August 2024 9,518 2 View

How to Compress Information Neurally?

Samuel Morse, the inventor of the Morse Code, understood that certain letters in the English language occurred more frequently than others (Gallistel and King 2010). To deal with this, Morse used...

01 August 2024 4,456 2 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Which transport medium is best for E.coli?

I'm working on Microbiology EQA program. I have confirmed E.coli strains in my lab. Now I want to transport them to other labs at a distance of 10-12 hrs. we don't want to use ice-packs during...

23 July 2024 1,855 0 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View