What is the function of sucrose in lysis buffers for isolation of intact nuclei? I observed that nuclei pellets resuspend easier in buffers without sucrose, and have a higher tendency to clump when sucrose (0.3M) is present. Clumping does not seem a good thing to me. So is there any other function of sucrose apart from being a density gradient medium for subsequent purification?

Most buffers would contain 0.25-0.3M sucrose. Why?

More Lech Kaczmarczyk's questions See All
Similar questions and discussions