Lech Kaczmarczyk

4 Questions 22 Answers 0 Followers

Questions related from Lech Kaczmarczyk

What are the possible ways to improve recombination efficacy of Cre- and Flp- dependent STOP cassetes?

Cre recombination efficiency often impacts activation of Cre/LoxP dependent transgenes. We have recently encountered problems with recombination in certain cell types in vivo, especially with...

12 August 2019 7,515 3 View

Can MeDIP-seq (methylated DNA immpunoprecipitation sequencing) be performed on DNA previously stained with DAPI or Hoechst?

Hi All, Can MeDIP-seq (methylated DNA immunoprecipitation-sequencing) be performed on DNA previously stained with DAPI or Hoechst? If not, are there any DNA stains compatible with the...

14 November 2016 2,571 1 View

What is the function of sucrose in lysis buffer for isolation of intact nuclei?

What is the function of sucrose in lysis buffers for isolation of intact nuclei? I observed that nuclei pellets resuspend easier in buffers without sucrose, and have a higher tendency to clump...

15 June 2016 8,470 10 View

What is the best strategy for FACS staining of nuclear protein for sorting fixed nuclei?

Hi All, I need to FACS-sort formaldehyde-fixed nuclei from mouse brain tissue. What is the best strategy for staining of intranuclear protein for immunolabeling for FACS (staining of soluble...

15 May 2016 5,169 3 View