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Questions related from Lech Kaczmarczyk
Cre recombination efficiency often impacts activation of Cre/LoxP dependent transgenes. We have recently encountered problems with recombination in certain cell types in vivo, especially with...
12 August 2019 7,525 3 View
Hi All, Can MeDIP-seq (methylated DNA immunoprecipitation-sequencing) be performed on DNA previously stained with DAPI or Hoechst? If not, are there any DNA stains compatible with the...
14 November 2016 2,587 1 View
What is the function of sucrose in lysis buffers for isolation of intact nuclei? I observed that nuclei pellets resuspend easier in buffers without sucrose, and have a higher tendency to clump...
15 June 2016 8,489 10 View
Hi All, I'm looking for an antibody directed against mouse nuclei (not cell type-specific), preferably directed to component(s) of nuclear envelope, and being a monoclonal. Antibody is intended...
07 June 2016 4,157 1 View
Hi All, I need to FACS-sort formaldehyde-fixed nuclei from mouse brain tissue. What is the best strategy for staining of intranuclear protein for immunolabeling for FACS (staining of soluble...
15 May 2016 5,184 3 View
Hi All, I was just wondering if anyone tried to use any of the Miltenyi beads in tubes instead of columns, with a standard magnets compatible with the "tube" magnetic separation format. My guess...
04 May 2016 275 3 View
Should using Taq polymerase be avoided when when doing SOE PCR? I would assume that, due to addition of non-template A and lack of exonuclease activity, it would generate mutant extension...
19 July 2015 4,111 5 View