Hi,
We have a question about the brain sample preparation for the kit. We have the supernatant in PE buffer from brain homogenization. The recipe of PE buffer (250 ml) is: 0.1 mM EDTA, 50 mM 85% phosphoric acid, and 50 uM methyldopa in polished water. This the leftover sample for dopamine, a neuropeptide in HPLC measurement. Methyldopa is used for internal control of HPLC.
Is this supernatant suitable for the IL-6 ELISA kit here? Can we add some chemical like 5 M guanidine HCL to the PE sample to modify it for the ELISA?
We have very limited and precious sample, so we’d like to see if we could use these leftover samples from HPLC.
Two main things could affect the ELISA results: the presence of detergent and the pH. You may have a problem because of the phosphoric acid. Check the pH!
Do use high sensitivity ELISA - for instance from R&D Systems. Think about using HPLC to detect IL6.
Good luck!
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