Waijiao Cai

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Questions related from Waijiao Cai

Is actin or any other housekeeping proteins a suitable loading control in WB in the sequential extraction samples?

Hello guys, I am recently doing WB to detect soluble and insoluble species of a protein using sequential extraction. I have a question about the loading control. Generally, we use...

10 October 2018 6,918 2 View

IL-6 ILISA detection in mice brain samples prepared from HPLC measurement?

Hi, We have a question about the brain sample preparation for the kit. We have the supernatant in PE buffer from brain homogenization. The recipe of PE buffer (250 ml) is: 0.1 mM EDTA, 50 mM 85%...

01 January 1970 2,816 2 View

WB detection of aSyn aggregates/oligomers /monomers in a AAV8-wt-human aSyn mouse model?

Hello, We are trying to detect the aSyn aggregates/oligomers /monomers in the AAV8-wt-aSyn mouse model. It is all about the technique details. For the biochemical extraction part, I am...

01 January 1970 9,154 3 View