26 September 2020 3 1K Report

I would like to extract membrane proteins of platelets, but I can't find more detailed protocols. How should I extract without a commercial kit?

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How to regulate Ransome in criminal law?

Ransomware attack has become a common criminal pattern, but the governance of countries basically focuses on fraud and computer crime. Dear colleagues, I would like to ask you how the definition...

21 May 2024 262 8 View

Why can't the precursor dissolve in THF after removing the end group by AIBN?

I prepare the precursor with both hydroxy and Nitrogenous group on the side chain by RAFT. Then, through the method mentained in the following literature that add AIBN to wipe off the residue of...

11 May 2024 2,941 0 View

Why does EVA alcoholysis product evoh fail to dissolve in DMSO when determining nuclear magnetism?

使用购买的EVA（VA含量40%）产品，在甲苯溶液中溶解，然后添加了氢氧化钠和醇的混合溶液，浓度大概有1mol/l和0.25mol/l的。 产品测试红外结果较为正常，但是在DMSO中一直无法溶解，困扰了很久。有没有做这方面研究的大佬可以解答一下或者讨论一下。是否自己合成的EVA再去做EVOH会好溶于dmso一些。

21 December 2023 7,565 0 View

Why does EVA alcoholysis product evoh fail to dissolve in DMSO when determining nuclear magnetism?

The purchased EVA (VA content 40%) product is dissolved in toluene solution, and then a mixture of sodium hydroxide and alcohol is added, the concentration is about 1mol/l and 0.25mol/l. The...

21 December 2023 8,974 3 View

What cause my DNA stuck at the top of my well and why are there 2 lines in between my smear??

I’m currently conducting research on telomeres in THP-1 cells. During my gel electrophoresis experiments using a 1% gel, I’ve encountered an issue where the DNA seems to be stuck at the top of the...

29 October 2023 9,561 7 View

Prepartion of styrene acrylic emulsion?

How to dissolve styrene-acrylic resin pellets(BASF Joncryl684) into a water/1,2-butanediol mixed solution to prepare an emulsion?

29 October 2023 2,745 0 View

Prepartion of styrene acrylic emulsion?

I dissolved the styrene acrylic resin (molecular weight 1500): 1,2-butanediol = 3:5（weight ratio）, added the emulsifier, and then slowly added it to the water containing the emulsifier using a...

27 October 2023 3,341 0 View

Target was not seen in the whole cell lysate but it was seen in the supernatant after IP?

I was recently doing a Co-IP work. And I got some weird result as show in the picture, the target protein was not seen in the whole cell lysate but it was seen in the supernatant after IP. The...

30 August 2023 7,220 0 View

I would like to ask a question about COMSOL coils, How to set up comsol to make it run?

I simulated a copper coil in comsol, applied a pulse excitation current to the coil, and set a probe around the coil to measure the potential. I used the mef module and transient research, but it...

03 July 2023 3,955 2 View

Is it possible to prepare polymethylmethacrylate from methacrylic acid and methanol monomers?

Is it possible to prepare polymethylmethacrylate from methacrylic acid and methanol monomers?

29 May 2023 2,903 1 View

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