I would like to extract membrane proteins of platelets, but I can't find more detailed protocols. How should I extract without a commercial kit?
A brief literature search will enable you to find papers describing methods for preparation of platelet membrane proteins. Here are a few examples:
https://www.jbc.org/content/247/14/4468.full.pdf
https://ashpublications.org/blood/article/114/1/e10/26099/Platelet-membrane-proteomics-a-novel-repository
Article Exploring the Potential of the Platelet Membrane Proteome as...
This can also be of help. Goodluck
Article Enrichment of Human Platelet Membranes for Proteomic Analysis
DOI: 10.1007/978-1-60327-310-7_17
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