Dear Dr. Cai,
my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL; https://www.wsl.ch/en/about-wsl/research-units/forest-health-and-biotic-interactions/phytopathology.html).
I am currently leading a project in which we aim at looking for viruses in the fungus Hymenoscyphus fraxineus, the causal agent of ash dieback in Europe. The idea behind this is to test if such viruses (if present) could be used for the biological control of the pathogen (as in the chestnut blight fungus Cryphonectria parasitica). For this study, we screen native populations of H. fraxineus in which chances to find viruses are highest. We currently have three populations from Japan, but it would be great if we could test additional populations from the native range of the species, in particular China, Korea and East Russia.
Would you have Asian isolates of H. fraxineus to share with us or do you know somebody else who may have such isolates?
Sorry for bothering you with this and many thanks in advance for your help!
I hope for a global overview on mathematical giftedness and its support in school and/or on an extracurricular level. What programmes/opportunities are offered?
08 July 2020 7,124 4 View
Following Gromacs Manual and other suggestions from different topics I create a molecule with Avogadro, I decided to employ oplsaa ff (I copied it in a local directory giving a new name) and I...
25 March 2020 4,438 3 View
Dear all, I am dealing with some crystal structure in which it is possible to replace Br- anions with Cl- anions. I am trying the refinement by setting the occupancy factor according to the molar...
24 February 2020 4,122 2 View
Hi, I have a question about the count of 3D spheroids. How can I count the number of spheroids starting from the number of seeded cells? After 3 days colture I count spheroids that have 100 um...
26 March 2018 1,596 3 View
We are having difficulty detecting PDCD4 in serum. We have ordered an ELISA to attempt this, but have not tried it yet. Afraid something is wrong with serum.
08 January 2018 4,637 3 View
I wanna know how I can measure or prove that a coating process was effective in nanoparticles. Some investigators have used the dynamic light scattering to prove it, but I think that if you change...
11 December 2017 4,333 6 View
I am having problems with high efficiency of my primers and one reason could be the formation of primer dimers. I dont see any big peak on the melt curve, but I dont know if this increased peak is...
19 September 2017 7,270 4 View
I have run RNA samples in 1% agarose gel but I cannot visually see a ratio 28S/18S ~ 2. What I might be doing wrong? Also, the samples marked with numbers, should I still consider them for qPCR?
12 September 2017 6,958 6 View
Hello,Can anyone suggest a bioinformatic approach to studying tumor heterogeneity in tumor samples? In other words, I performed exome analysis on my samples and now I want to build an evolutionary...
19 July 2017 1,475 1 View
I am searching for a good place for the Post Doc,
02 March 2021 4,053 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...
01 March 2021 7,675 3 View
Incompletely cross-linked pdms are difficult to remove.
01 March 2021 798 3 View
Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...
28 February 2021 7,356 8 View
The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.
28 February 2021 9,464 2 View
The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...
28 February 2021 6,560 14 View
Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...
28 February 2021 9,138 2 View
Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.
27 February 2021 9,356 3 View
Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...
26 February 2021 4,026 5 View