What PCR conditions for a matk (universal primer)with Plant genomic DNA as template is suitable?

Conditions we used were- 98deg 45s

03 April 2017 10,000 5 View

What is the binomial name of buddha bamboo(nodulated bamboo)?

01 February 2017 5,780 0 View

What are the mistakes or errors that i have committed for this pcr?

we have extracted dna by ctab method from tender leaves this is for 10 microliter reaction why did we get multiple bands for rbcl? Kindly provide suggestions to improve my work by viewing the...

11 December 2016 4,881 9 View

Among the buffers which one is most suitable for plant genomic dna extraction by ctab method, TBE OR TAE?

Which buffer would suitable for dna extraction of bamboo species ? If so what would be the reason?

08 September 2016 9,824 5 View

When I am extracting the DNA by CTAB Method form bamboo leaves, the pellet which is obtained at last step when mixed with TE Buffer becomes slimy?

Why does it becomes slimy?I initally diluted it to 30micro litre, then again i redissolved in 50, but its still slimy. I would like to go for PCR after the extraction process. Any suggestions to...

08 September 2016 2,582 5 View

For green synthesis of AgNO3 nanoparticles, should the UV Spectrum must have sharp peaks in and around 420nm or broadened peaks(dome like )?

I had a discussion with a researcher in an conference who did not have sharp peak but was dome shaped, she justified the reason for the dome shaped nature because the nano particles were well...

07 August 2016 5,763 8 View

Why is half strength media used for rooting of in vitro cultures and not full strength?

Rooting of invitro plant cultures.

05 June 2016 7,552 10 View

A journal is indexed in http://ip-science.thomsonreuters.com/, but in thomson reuters2016 journal list its name is not given. Why is it so?

The name of journal is Journal of Pure and Applied Microbiology. What is the status of the journal whether its TR OR NOT? IT is INDEXED IN WEB OF SCIENCE.

05 June 2016 5,194 4 View

Why is isopropanal ice cold and mortar and pestle pre-chilled during dna extraction?

For genomic dna extraction from plants

04 May 2016 4,925 3 View

When is the next flowering year or season for Dendrocalamus strictus and Bambusa vulgaris?

I have heard that bamboo plants die soon after flowering, so would like to know the time period of their flowering. Please help. Thanks

02 March 2016 1,856 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How to add a journal?

I would like to know how to register the journal Flora Montiberica (ISSN 1138-5952 - eISSN 1988-799X) in Research Gate.

02 March 2021 8,156 2 View

Is it possible to remove a preprint posted by a journal which is already rejected?

As we know many journals are posting preprints (DOI) in advance before the peer-review process. However, there is no guarantee that the preprints get published. The journal's reviewers undergo the...

01 March 2021 5,778 2 View

How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

No title

Hi, I have several doubts about one paper (Systematic Literature Review) that was rejected. The main topic is Single-Board Computers in engineering education during the period 2010-2020. The...

01 March 2021 9,015 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View