What PCR conditions for a matk (universal primer)with Plant genomic DNA as template is suitable?

Conditions we used were- 98deg 45s

03 April 2017 10,000 5 View

What is the binomial name of buddha bamboo(nodulated bamboo)?

01 February 2017 5,780 0 View

What are the mistakes or errors that i have committed for this pcr?

we have extracted dna by ctab method from tender leaves this is for 10 microliter reaction why did we get multiple bands for rbcl? Kindly provide suggestions to improve my work by viewing the...

11 December 2016 4,881 9 View

Among the buffers which one is most suitable for plant genomic dna extraction by ctab method, TBE OR TAE?

Which buffer would suitable for dna extraction of bamboo species ? If so what would be the reason?

08 September 2016 9,824 5 View

When I am extracting the DNA by CTAB Method form bamboo leaves, the pellet which is obtained at last step when mixed with TE Buffer becomes slimy?

Why does it becomes slimy?I initally diluted it to 30micro litre, then again i redissolved in 50, but its still slimy. I would like to go for PCR after the extraction process. Any suggestions to...

08 September 2016 2,582 5 View

For green synthesis of AgNO3 nanoparticles, should the UV Spectrum must have sharp peaks in and around 420nm or broadened peaks(dome like )?

I had a discussion with a researcher in an conference who did not have sharp peak but was dome shaped, she justified the reason for the dome shaped nature because the nano particles were well...

07 August 2016 5,763 8 View

Why is half strength media used for rooting of in vitro cultures and not full strength?

Rooting of invitro plant cultures.

05 June 2016 7,552 10 View

Why is isopropanal ice cold and mortar and pestle pre-chilled during dna extraction?

For genomic dna extraction from plants

04 May 2016 4,925 3 View

When is the next flowering year or season for Dendrocalamus strictus and Bambusa vulgaris?

I have heard that bamboo plants die soon after flowering, so would like to know the time period of their flowering. Please help. Thanks

02 March 2016 1,856 1 View

I wrote a review paper on contamination in plant tissue culture, can any one suggest me journals for review paper?

I am Ph.d scholar, I would like to get journals with low publication fees Kindly help

31 December 2015 1,335 0 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View