Leelavathy Suresh

13 Questions 17 Answers 0 Followers

Questions related from Leelavathy Suresh

What PCR conditions for a matk (universal primer)with Plant genomic DNA as template is suitable?

Conditions we used were- 98deg 45s

04 April 2017 10,072 5 View

What is the binomial name of buddha bamboo(nodulated bamboo)?

Whats the difference between bambusa vulgaris warmin and bambusa ventricosa? Both google images are having nodulations in stem.

02 February 2017 5,850 0 View

What are the mistakes or errors that i have committed for this pcr?

we have extracted dna by ctab method from tender leaves this is for 10 microliter reaction why did we get multiple bands for rbcl? Kindly provide suggestions to improve my work by viewing the...

12 December 2016 4,951 9 View

When I am extracting the DNA by CTAB Method form bamboo leaves, the pellet which is obtained at last step when mixed with TE Buffer becomes slimy?

Why does it becomes slimy?I initally diluted it to 30micro litre, then again i redissolved in 50, but its still slimy. I would like to go for PCR after the extraction process. Any suggestions to...

09 September 2016 2,653 5 View

Among the buffers which one is most suitable for plant genomic dna extraction by ctab method, TBE OR TAE?

Which buffer would suitable for dna extraction of bamboo species ? If so what would be the reason?

09 September 2016 9,899 5 View

For green synthesis of AgNO3 nanoparticles, should the UV Spectrum must have sharp peaks in and around 420nm or broadened peaks(dome like )?

I had a discussion with a researcher in an conference who did not have sharp peak but was dome shaped, she justified the reason for the dome shaped nature because the nano particles were well...

08 August 2016 5,838 8 View

A journal is indexed in http://ip-science.thomsonreuters.com/, but in thomson reuters2016 journal list its name is not given. Why is it so?

The name of journal is Journal of Pure and Applied Microbiology. What is the status of the journal whether its TR OR NOT? IT is INDEXED IN WEB OF SCIENCE.

06 June 2016 5,287 4 View

Why is half strength media used for rooting of in vitro cultures and not full strength?

Rooting of invitro plant cultures.

06 June 2016 7,642 10 View

Why is isopropanal ice cold and mortar and pestle pre-chilled during dna extraction?

For genomic dna extraction from plants

05 May 2016 5,058 3 View

When is the next flowering year or season for Dendrocalamus strictus and Bambusa vulgaris?

I have heard that bamboo plants die soon after flowering, so would like to know the time period of their flowering. Please help. Thanks

03 March 2016 1,925 1 View

I wrote a review paper on contamination in plant tissue culture, can any one suggest me journals for review paper?

I am Ph.d scholar, I would like to get journals with low publication fees Kindly help

01 January 2016 1,397 0 View

Can reuse bacteria contaminated plants it by treating it with sterilizing chemicals or is It to be discarded?

I used bamboo axillary nodes as explants, after inoculation of 10 days bacterial contamination was identified, shall I reuse the plant by giving chemical treatment or should it be discarded?

03 March 2015 9,307 13 View

Where can I get certified bamboo seeds from Tamilnadu, Kerala, Bangalore?

I need these quickly.

12 December 2014 7,159 5 View