Which buffer would suitable for dna extraction of bamboo species ?
If so what would be the reason?
Hi!
First make your question a bit clear. Buffer ingredients are as follows
CTAB buffer: 2% cetyl trimethylammonium bromide, 1% polyvinyl pyrrolidone,100 mM Tris-HCl, 1.4 M NaCl, 20 mM EDTA,
Why the question TAE/TBE come here?
Or once you isolate the DNA to check that you need to run the gel, right. For that it is your choice you can use any buffer. There is nothing compulsory that you should use TAE only or only TBE. It is your choice. Some people use TAE some use TBE.
Sorry ma'am, I would like to know abt the tank buffers..I want to know the difference and need of both TAE and TBE,,Does it have any connection with the DNA size..?
Hello. For what purposes you want to extract your DNA? If you want to do only PCR , I had best done it by quick extraction. I can send you the protocol which was good for N.tabacum and B.napus. I had examined a lot of protocols but the last one was the best.
Yes dear I already answered your question that so called tank buffers only 'need to run the gel' (Electrophoresis) that I mentioned in earlier answer. Seems still you are not satisfied with my answer. I said you can use any buffer some labs prepare TAE some prepare TBE or both (choice is yours). Generally buffers are to pass/conduct electricity so that your DNA/Protein moves.
Otherwise read the book 'Molecular cloning : a laboratory manual / J. Sambrook, E.F. Fritsch, T. Maniatis'. Very good book for Mol. Bio. protocals and you get sound knowledge. I believe all labs possess this. Read it and use what ever the buffer you like.
The difference is 'TAE has a lower buffer capacity than TBE and can easily become exhausted, but linear, double stranded DNA runs faster in TAE.'
'TBE or Tris/Borate/EDTA, is a buffer solution containing a mixture of Tris base, boric acid and EDTA.
TAE buffer is a buffer solution containing a mixture of Tris base, acetic acid and EDTA.'
Dear Ma'am,
Thank you ma'am. I will definitely go through it.
@Mahdieh Mehrab Mohseni
I am doing PCR of the samples.
Kindly send me the protocol that you have mentioned.
thank you for the help.
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