What PCR conditions for a matk (universal primer)with Plant genomic DNA as template is suitable?

Conditions we used were- 98deg 45s

03 April 2017 10,000 5 View

What is the binomial name of buddha bamboo(nodulated bamboo)?

01 February 2017 5,780 0 View

What are the mistakes or errors that i have committed for this pcr?

we have extracted dna by ctab method from tender leaves this is for 10 microliter reaction why did we get multiple bands for rbcl? Kindly provide suggestions to improve my work by viewing the...

11 December 2016 4,881 9 View

Among the buffers which one is most suitable for plant genomic dna extraction by ctab method, TBE OR TAE?

Which buffer would suitable for dna extraction of bamboo species ? If so what would be the reason?

08 September 2016 9,824 5 View

When I am extracting the DNA by CTAB Method form bamboo leaves, the pellet which is obtained at last step when mixed with TE Buffer becomes slimy?

Why does it becomes slimy?I initally diluted it to 30micro litre, then again i redissolved in 50, but its still slimy. I would like to go for PCR after the extraction process. Any suggestions to...

08 September 2016 2,582 5 View

For green synthesis of AgNO3 nanoparticles, should the UV Spectrum must have sharp peaks in and around 420nm or broadened peaks(dome like )?

I had a discussion with a researcher in an conference who did not have sharp peak but was dome shaped, she justified the reason for the dome shaped nature because the nano particles were well...

07 August 2016 5,763 8 View

Why is half strength media used for rooting of in vitro cultures and not full strength?

Rooting of invitro plant cultures.

05 June 2016 7,552 10 View

A journal is indexed in http://ip-science.thomsonreuters.com/, but in thomson reuters2016 journal list its name is not given. Why is it so?

The name of journal is Journal of Pure and Applied Microbiology. What is the status of the journal whether its TR OR NOT? IT is INDEXED IN WEB OF SCIENCE.

05 June 2016 5,194 4 View

Why is isopropanal ice cold and mortar and pestle pre-chilled during dna extraction?

For genomic dna extraction from plants

04 May 2016 4,925 3 View

I wrote a review paper on contamination in plant tissue culture, can any one suggest me journals for review paper?

I am Ph.d scholar, I would like to get journals with low publication fees Kindly help

31 December 2015 1,335 0 View

What would be the best method to study the seasonal variation in wetland vegetation?

I wanted to learn how the seasonal variation relate to climate change. The best samplimg methods are required. Therefore, any references or suggestions are welcome. Thank you

24 February 2021 8,589 3 View

What is this fern?

Please help me to identify this fern species. Thank you in advance.

24 February 2021 2,435 5 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

Can medicinal plants really play an inherent rule in the COVID-19 tackle?

Numerous scientific reports cited plant extracts with potential antiviral activity and even anti-COVID-19? Why these findings are neglected? I remember very well the warning released by the WHO to...

17 February 2021 2,141 9 View

Is there any solar electric power plant having the concentrated photovoltaic modules?

The solar photovoltaic (PV) power plant uses commercial (non-concentrated) PV modules which are quite simple in design and reliable. They can work on fixed orientation and don't need the moving...

16 February 2021 3,796 9 View

Does the subsoil have an effect on the spread of weeds whose roots are more than 35 cm deep?

For weeds whose roots deepen more than the level of subsoil, they may be affected by this layer, for example, determined by height and growth, as well as limited to flower production and seeds.

01 February 2021 1,940 4 View

Is it possible estimating environmental conditions with a Bayesian approach?

In ecology specific species can be used to get an indication/estimation of the environmental conditions. A simplistic example, lets assume I find a pike (Esox lucius). We know this species does...

01 February 2021 10,034 4 View

How can a traditional medicine exist if its validated outcome is translated into molecular mechanism for acceptance of modern medicine?

A traditional medicine (e.g. Unani System of Medicine) has its own philosophy of treatment of a disease. Nowadays, there is emerging demand to validate the effectiveness of an intervention (a...

05 January 2021 9,472 5 View

How yo prepare samples for RNA extraction?

I really need help. I tried finding a protocol on how to prepare samples for gene expression . I am working on Antimicrobial activity of a medicinal plant and I would like to check its effect on...

30 December 2020 7,112 2 View

What is the level of consciousness , communication and feeling in plants ?

Do you think the plant can communicate with each other, what is the level of feeling in plants?

22 December 2020 2,754 17 View