Can someone suggest simple protocols for protein clonning in Ecoli and how to express gene/protein?

i want to want on protein clonning by using Ecoli as host, very preliminary training i need for this. can someone suggest protocols for simple techniques for clonning, and also simple protocols...

05 June 2016 1,103 2 View

Protocols for energy production from bacteria

02 March 2016 7,541 2 View

In uv-vis characterization of silver nanoparticles synthesized by plant extract at what position should peak comein the 300 nm to 700 nm range?

11 December 2015 876 15 View

Silver nanoparticles give surface plasmon response at 400 to 470 nm but my extracts have been giving peak at 320-380 nm .Is it correct or not?

i m working on synthesis of silver nanopraticles by plant extract by using 1mM AgNO3 . these extracts is giving peak at 310-350 nm ,,, i have selected the 300 to 700 nm range. should i work on...

11 December 2015 8,378 16 View

Role of protein kinases in cancer

What are the role of protein kinases in cancer?

05 June 2012 7,397 9 View

I need some enzyme assay for soil urease activity using sodium hypochlorite

Actually i m working on soil urease and stuck at the step of identification of urease activity after ammunium sulphate saturation .i am using sodium hypochlorite rex but i dont know how graph is...

31 December 2011 8,544 14 View

I ve some problems in puritfication of papain from papayya

I need some research paper on purificatiom of papain from papayya fruit

31 December 2011 5,788 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View