Can someone suggest simple protocols for protein clonning in Ecoli and how to express gene/protein?

i want to want on protein clonning by using Ecoli as host, very preliminary training i need for this. can someone suggest protocols for simple techniques for clonning, and also simple protocols...

05 June 2016 1,103 2 View

In uv-vis characterization of silver nanoparticles synthesized by plant extract at what position should peak comein the 300 nm to 700 nm range?

11 December 2015 876 15 View

Silver nanoparticles give surface plasmon response at 400 to 470 nm but my extracts have been giving peak at 320-380 nm .Is it correct or not?

i m working on synthesis of silver nanopraticles by plant extract by using 1mM AgNO3 . these extracts is giving peak at 310-350 nm ,,, i have selected the 300 to 700 nm range. should i work on...

11 December 2015 8,378 16 View

Role of protein kinases in cancer

What are the role of protein kinases in cancer?

05 June 2012 7,397 9 View

I need some enzyme assay for soil urease activity using sodium hypochlorite

Actually i m working on soil urease and stuck at the step of identification of urease activity after ammunium sulphate saturation .i am using sodium hypochlorite rex but i dont know how graph is...

31 December 2011 8,544 14 View

I ve some problems in puritfication of papain from papayya

I need some research paper on purificatiom of papain from papayya fruit

31 December 2011 5,788 0 View

I want some detailed material about dna sequencing

Actually i want some research papers on dna sequencing and detail having reaction,separation,detection

11 December 2011 6,436 7 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View