Protocols for energy production from bacteria

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Can someone suggest simple protocols for protein clonning in Ecoli and how to express gene/protein?

i want to want on protein clonning by using Ecoli as host, very preliminary training i need for this. can someone suggest protocols for simple techniques for clonning, and also simple protocols...

05 June 2016 1,149 2 View

In uv-vis characterization of silver nanoparticles synthesized by plant extract at what position should peak comein the 300 nm to 700 nm range?

in the biosynthesis of silver nanoparticles from plant by using 1mM AgNO3 two peaks at 668 and 330 nm had observed. at 0ml of plant extract 668 nm had be observed . i m confused about which peak...

11 December 2015 987 15 View

Silver nanoparticles give surface plasmon response at 400 to 470 nm but my extracts have been giving peak at 320-380 nm .Is it correct or not?

i m working on synthesis of silver nanopraticles by plant extract by using 1mM AgNO3 . these extracts is giving peak at 310-350 nm ,,, i have selected the 300 to 700 nm range. should i work on...

11 December 2015 8,428 16 View

Role of protein kinases in cancer

What are the role of protein kinases in cancer?

05 June 2012 7,443 9 View

I need some enzyme assay for soil urease activity using sodium hypochlorite

Actually i m working on soil urease and stuck at the step of identification of urease activity after ammunium sulphate saturation .i am using sodium hypochlorite rex but i dont know how graph is...

31 December 2011 8,607 14 View

I ve some problems in puritfication of papain from papayya

I need some research paper on purificatiom of papain from papayya fruit

31 December 2011 5,858 0 View

I want some detailed material about dna sequencing

Actually i want some research papers on dna sequencing and detail having reaction,separation,detection

11 December 2011 6,476 7 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to develop investments in renewable energy sources?

08 August 2024 5,112 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Deepak Pant

In case you want to work with microbial fuel cells, you may refer to the following papers, which give a protocol on how to work with them in detail-

1/ http://www.sciencedirect.com/science/article/pii/S073497501500052X

2/ http://pubs.acs.org/doi/abs/10.1021/es0605016

3/ https://books.google.com.qa/books?hl=en&lr=&id=cXnc2wmrE9gC&oi=fnd&pg=PR7&dq=info:r_GcrC2H8bAJ:scholar.google.com&ots=CmXV1TBmfK&sig=eUdWSsoGGQRcWCW6LUtYm4KibOU&redir_esc=y#v=onepage&q&f=false

Rabia Shafiq

thanks for your help. but i need some research articals