Can someone suggest simple protocols for protein clonning in Ecoli and how to express gene/protein?

i want to want on protein clonning by using Ecoli as host, very preliminary training i need for this. can someone suggest protocols for simple techniques for clonning, and also simple protocols...

05 June 2016 1,103 2 View

Protocols for energy production from bacteria

02 March 2016 7,541 2 View

In uv-vis characterization of silver nanoparticles synthesized by plant extract at what position should peak comein the 300 nm to 700 nm range?

11 December 2015 876 15 View

Silver nanoparticles give surface plasmon response at 400 to 470 nm but my extracts have been giving peak at 320-380 nm .Is it correct or not?

i m working on synthesis of silver nanopraticles by plant extract by using 1mM AgNO3 . these extracts is giving peak at 310-350 nm ,,, i have selected the 300 to 700 nm range. should i work on...

11 December 2015 8,378 16 View

Role of protein kinases in cancer

What are the role of protein kinases in cancer?

05 June 2012 7,397 9 View

I need some enzyme assay for soil urease activity using sodium hypochlorite

Actually i m working on soil urease and stuck at the step of identification of urease activity after ammunium sulphate saturation .i am using sodium hypochlorite rex but i dont know how graph is...

31 December 2011 8,544 14 View

I want some detailed material about dna sequencing

Actually i want some research papers on dna sequencing and detail having reaction,separation,detection

11 December 2011 6,436 7 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to solve error "Cannot find position restraint file restraint.gro (option -r)." on GROMACS?

Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...

01 February 2021 780 3 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

Any stable dye to color acrylamide hydrogel beads?

Hi! We are generating hydrogel beads(50-60um) using microfluidics as single-cell beads. To simply all the experiments (especially the centrifuge), I am looking for a dye. The dye should be stable...

14 January 2021 4,383 8 View

How to phosphorylate specific residues on pymol?

Hi everyone, I am using the pyTMs plug in on Pymol to phosphorylate a particular threonine in my protein. I am having difficulty in selecting only one or two residues - it seems I can only...

05 January 2021 230 3 View