i want to want on protein clonning by using Ecoli as host, very preliminary training i need for this. can someone suggest protocols for simple techniques for clonning, and also simple protocols for gene and protein expression .
well, theory is one part and there are textbooks published on this topic. But starting in the lab without having someonew ith experience around is something I would not recommend. Isn't there anybody in your lab that could give you a proper training?