I transformed K.Lactis using kit(NEB #E1000S), and grow wild type control on selective media and saw growth. Why the untransformed yeasts are growing?

More Madina Baglanova's questions See All

Is it possible to conduct a meta-analysis for a single group in the REVMAN program? If so, could you please provide guidance on where to find instruc?

Is it possible to conduct a meta-analysis for a single group in the REVMAN program? If so, could you please provide guidance on where to find instructions for conducting this type of meta-analysis?"

14 September 2023 5,522 1 View

The methods of synthesis Polypyrrole?

I have only one information, that I need to use 0.05M pyrrole and 1M potassium nitrate. Could you describe step by step process of synthesis?

23 October 2022 7,883 1 View

How can i find Undergraduate Stress Questionnaire?

Dear colleagues, hello! Does anyone have the full text of the Undergraduate Stress Questionnaire (USQ, Crandall, Preisler & Aussprung) and manual for its use and interpretation? I would be...

05 November 2019 5,580 2 View

What are some good journals or conference series to publish environmental research works?

In details, if a tool is made which will be beneficial at the time of calamities and the tool is tested in real life where the outcome is significant.

18 October 2019 7,724 3 View

What are the fast readouts for the mouse exocrine pancreatic activity in vivo?

Hello everyone. I'm studying endocrine pancreas, but for some experimental studies I would like to also measure exocrine activity. Since exocrine activity is not my main focus and I have...

22 May 2018 6,029 2 View

What dose of sulfonylureas (Tolbutamide, Glibenclamide) should be used for acute IN VIVO injections?

I'm trying to activate beta cells in vivo acutely with Tolbutamide. This might sound trivial, but I found limited literature on the in vivo use of Tolbutamide. The paper below reports use of 20ul...

09 April 2018 841 0 View

CST high resolution images?

Dear AllThis has been a matter of concern to me and I am sure to many of us, When we design a microwave structure in CST we plot current or E/H field distribution on the structure. Now we often...

23 August 2017 9,417 2 View

Could you please recommend me any panel datasets about Internet freedom like the Freedom on the Net index, but with more countries than 65?

I want to apply data for research purposes. I would like to see the relationships between natural resources abundance and its impact on the Internet regulation policies/freedom in...

29 January 2017 7,949 2 View

Comsol metamaterial absorber

Hello everyone, If anybody working on metamaterials using comsol multiphysics software can plz share their file? I did my work on CST but for the validation part, I have to use comsol. It would...

01 January 2017 10,064 4 View

How to find Drude Lorentz constant parameters for chiral single walled carbon nanotube in optical region ?

Hello everyone, I need to find the Drude Lorentz constant parameters for single walled carbon nanotube in optical region for different chiralities. I found one paper in the literature in which...

28 July 2016 8,686 1 View

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Some new emerging problems on application of RL for scheduling in IoT networks?

I have seen plenty of existing works on applied Reinforcement Learning (RL) policies for optimized scheduling in IoT networks including Q-learning, DQNs, and the newer ones including PPO for...

01 August 2024 8,754 2 View

How to extract RNA from neurons?

Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...

23 July 2024 6,783 1 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

Use of NC or NTC to descibe negative control?

Hello, I would like to ask which is the best terminology to use for describing a negative control in an experiment (drug testing in-vitro, viral infection): NC (negative control) or NTC? Thank you...

18 July 2024 4,183 2 View

I have constucted a plasmid vector from puc19 with no antibiotic and lacz gene. The problem is screening for transformed cells. Any suggestions?

Get too many colonies, which are mostly non-transformants when tested by colony PCR

11 July 2024 6,775 3 View

Cross Attention in Transformers: Standard applications of the same ?

What are the standard applications of Cross Attention in Transformer Architectures ?

09 July 2024 9,310 2 View

Solvent recovery after extraction - Separation of Isopropanol + Water - how to do it best?

I want to make some yeast extractions with a protocol that uses Isopropanol and NaOH. So in the end i will have an azeotrope of Isopropanol and Water that comes from the cell and NaOH. Can you...

08 July 2024 9,201 2 View