PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Please let me know how to remove mycoplasma contamination from cell line?

I am facing a problem in maintaining the cell line of HepG2 and HepG2.2.15. I have used plasmocin Tm treatment (Invivo gen) Cat.#ant-mpt Lot #MPT-41-01 for more than a month. I have also used...

21 February 2021 6,046 4 View

Western blot protein of interest falling at a lower weight than expected?

My protein of interest is DAGLa (115 kDa). We received the antibody for this from another researcher who reliably shows bands at this weight. We do see a light banding at this weight, but the...

11 February 2021 6,009 3 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

Sample Size for Quasi-Experimental Quants using one-tailed t-test?

Hi, sorry I'm stumped. I'm going to do a study on the leadership styles (using the MLQ Form 5X) of minority women administrators in medical schools. How will I calculate the sample size? Thank...

30 January 2021 2,865 4 View

Can you do Ponceau staining after loading control for Western Blot?

Hello everyone! I forgot to take a picture of my Ponceau stain prior to starting my first round of primary and secondary antibodies. I'm currently doing my loading control now and was wondering...

25 January 2021 757 3 View

What is the correct way to apply a Design effect for sample size calculation when the sampling method is linear systematic random sampling?

When we are doing sampling by means of simple systematic random sampling,how do we calculate the design effect for use during calculation of sample size for our work?

20 January 2021 5,328 3 View

Anybody to help me with Code source for DOUBLE CONCAVE FRICTION PENDULUM BEARINGS Please?

Hello, would you please share the codes for Base Isolation of structures using Double Concave Friction Pendulum Bearings? I am doing a related research but as I am just a beginner in MATLAB, I...

18 January 2021 6,683 1 View

How to generate .dlg files on PyRx?

Hi. I'm using the PyRx version 0.8 and getting the error message: WARNING: Filename mismatch: "2DFN_ADP_CL.maps.fld" :: "..\2DFN_ADP_CL.maps.fld" C:\Program Files (x86)\The Scripps Research...

17 January 2021 843 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

What could be the cause to have an E.coli DH10B transformed with pUC18 to grow in MKL + ampicillin but is not LAC+?

Hello everyone, I did a transformation of E. coli DH10B using pUC18 plasmid. The bacterias did grow on MKL medium with ampicillin but the colonies showed a white/pink colour and the medium was...

08 February 2021 9,720 3 View

Can I know how to apply contourlet transform for signals in MATLAB?Must I first convert 1D signal to 2D image in order to do this method?

Can I know how to apply contourlet transform for signals in MATLAB?Must I first convert 1D signal to 2D image in order to do this method?

01 February 2021 3,735 3 View

What are the pretrained-language-model that is obviously better than BERT and RoBERTa?

19 January 2021 602 2 View

WHOQOL - bref. test: how to conduct the correction?

18 January 2021 7,257 2 View

How to compute the phase from a fringe pattern using Hilbert Huang Transform in MATLAB?

I have computed line Wise IMF from the fringe data using empirical mode decomposition (EMD). For each line Segment I have more than five IMF sets but i am not able to understand that how to...

10 January 2021 5,036 1 View

Which statistical test is best to run on a heavily right-skewed dependent variable?

My dependent variable when graphed is heavily right-skewed. All attempts to transform it has failed (Log, Log10, Sqrt, ..). The most ideal test I would like to run is a multiple linear...

07 January 2021 2,705 4 View

How can I estimate and plot Logarithmic Error Bars (in Excel, preferably)?

Dear Dr.colleagues, I am currently plotting the dynamics of an infection in Excel. For each sampling day, I plotted the mean infection load of the population, using the standard error for the...

07 January 2021 2,069 3 View

Why I am not getting cultures of DH5 alpha and BL21 cells after transformation?

Even if I get them cultured colonies are very less and almost not transferable and grows very slowly in the media. I checked cells by transforming with control vector. I changed antibiotic also...

06 January 2021 1,013 3 View

How i can fix this problem for python jupyter" Unable to allocate 10.4 GiB for an array with shape (50000, 223369) and data type int8"?

my code: #building tf-idf from sklearn.feature_extraction.text import TfidfVectorizer vectorizer = TfidfVectorizer(analyzer = message_cleaning) #X = vectorizer.fit_transform(corpus) X =...

28 December 2020 3,624 2 View