Madina Baglanova

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Questions related from Madina Baglanova

How to Isolate Intracellular Bacteria from Epithelial Cells Without Host Contamination?

Dear colleagues, I am isolating intracellular bacteria from epithelial cells but keep encountering host cell contamination. My protocol: Lysis: PBS wash → 0.1% Triton X-100 (10 min at 37°C, 20 min...

31 January 2025 7,016 0 View

I transformed K.Lactis using kit(NEB #E1000S), and grow wild type control on selective media and saw growth. Why the untransformed yeasts are growing?

The media that was used YCB?

05 September 2023 8,840 2 View