Does anyone know a substitute for SNAP?

Hi, I have a list of SNPs and I wan t to find the SNPs which are in the same LD blocks with them. Does anyone know a software or an online tool for that? Thanks

06 July 2018 7,441 3 View

How to calculate Power and OR in genetic association studies?

In studying the effect of a SNP on susceptibility to a disease in a case-control design, How I can calculate the detectable OR for a given sample size with a power of 80%? which parameters I need...

01 February 2016 7,704 5 View

How can I analyse the data from a single SNP under additive mode?

I want to analyse my data to see if there is an association between a disease and an SNP, should I use an additive model? How can I perform the analysis using R or SPSS?

06 July 2014 5,789 23 View

How can I analyse the results in R, when I have the allele frequencies of case and control groups?

I have the allele frequencies of both case and control groups, and I want to see if the allele frequencies are significantly different or not, I want to do the analysis in R.

04 May 2014 7,843 6 View

No title

04 May 2014 1,447 2 View

Anyone have any insight as to why DNA doesn't dissolve in TE?

I use a salting out method to isolate DNA from blood. Sometimes when I add TE to the participated DNA, it doesn't dissolve in it, even after one day. does anyone know why?

01 February 2014 3,018 4 View

Is it possible to isolate buffy coat from frozen blood?

We have some frozen blood in -20 freezer. They are there for about 1-2 months. Now I need to isolate WBCs. Does anyone know of an experiment I can use? I need them for DNA extraction.

01 February 2014 8,514 23 View

Does DNA get any damage if left dried without adding water or TE for some days after DNA extraction?

Will it be okay for experiments like PCR after dissolve in TE?

09 October 2013 7,718 6 View

Brown color DNA in salting out DNA extraction method - what's the problem?

I use a simple salting out method to extract DNA from blood, it usually works well, but in some samples the extracted DNA has a brown color and surprisingly the DNA doesn't dissolve in TE. Does...

09 October 2013 889 3 View

I have to perform a PCR by a reverse primer which have a mismatch, one nucleotide before the 3' end. How could it affect my PCR?

Do I have a big problem?

11 December 2012 2,756 26 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View