I need to extract DNA from buccal swabs of babies, does anyone have an experience with some protocol, extraction kit or something?

More Javad Jamshidi's questions See All

Does anyone know a substitute for SNAP?

Hi, I have a list of SNPs and I wan t to find the SNPs which are in the same LD blocks with them. Does anyone know a software or an online tool for that? Thanks

06 July 2018 7,603 3 View

How to calculate Power and OR in genetic association studies?

In studying the effect of a SNP on susceptibility to a disease in a case-control design, How I can calculate the detectable OR for a given sample size with a power of 80%? which parameters I need...

01 February 2016 8,003 5 View

How can I analyse the data from a single SNP under additive mode?

I want to analyse my data to see if there is an association between a disease and an SNP, should I use an additive model? How can I perform the analysis using R or SPSS?

06 July 2014 6,086 23 View

How can I analyse the results in R, when I have the allele frequencies of case and control groups?

I have the allele frequencies of both case and control groups, and I want to see if the allele frequencies are significantly different or not, I want to do the analysis in R.

04 May 2014 8,133 6 View

How can we analyze results when allele frequencies are not in Hardy–Weinberg equilibrium?

How should we analyze the results of a study detecting an association between as SNP and a disease, when the allele frequencies are not in Hardy–Weinberg equilibrium?

04 May 2014 1,783 2 View

Anyone have any insight as to why DNA doesn't dissolve in TE?

I use a salting out method to isolate DNA from blood. Sometimes when I add TE to the participated DNA, it doesn't dissolve in it, even after one day. does anyone know why?

01 February 2014 3,318 4 View

Is it possible to isolate buffy coat from frozen blood?

We have some frozen blood in -20 freezer. They are there for about 1-2 months. Now I need to isolate WBCs. Does anyone know of an experiment I can use? I need them for DNA extraction.

01 February 2014 8,818 23 View

Does DNA get any damage if left dried without adding water or TE for some days after DNA extraction?

Will it be okay for experiments like PCR after dissolve in TE?

09 October 2013 7,996 6 View

Brown color DNA in salting out DNA extraction method - what's the problem?

I use a simple salting out method to extract DNA from blood, it usually works well, but in some samples the extracted DNA has a brown color and surprisingly the DNA doesn't dissolve in TE. Does...

09 October 2013 976 3 View

I have to perform a PCR by a reverse primer which have a mismatch, one nucleotide before the 3' end. How could it affect my PCR?

Do I have a big problem?

11 December 2012 3,039 26 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Najme Alibabaei

You can use an organic protocol or chelex method.

Search and get a good protocol..

Amr M Ghaleb

Try using QIAamp DNA Mini Kit (50) from Qiagen.

Caitlin Muse

I use either the QIAamp Mini Kit from Qiagen or a simple method of heating to lyse and then neutralizing. Depends on what purity is needed. Also, Zymo DNA Clean and Concentrator is good and easy.