Does anyone know a substitute for SNAP?

Hi, I have a list of SNPs and I wan t to find the SNPs which are in the same LD blocks with them. Does anyone know a software or an online tool for that? Thanks

06 July 2018 7,441 3 View

How to calculate Power and OR in genetic association studies?

In studying the effect of a SNP on susceptibility to a disease in a case-control design, How I can calculate the detectable OR for a given sample size with a power of 80%? which parameters I need...

01 February 2016 7,704 5 View

How can I analyse the data from a single SNP under additive mode?

I want to analyse my data to see if there is an association between a disease and an SNP, should I use an additive model? How can I perform the analysis using R or SPSS?

06 July 2014 5,789 23 View

How can I analyse the results in R, when I have the allele frequencies of case and control groups?

I have the allele frequencies of both case and control groups, and I want to see if the allele frequencies are significantly different or not, I want to do the analysis in R.

04 May 2014 7,843 6 View

No title

04 May 2014 1,447 2 View

Anyone have any insight as to why DNA doesn't dissolve in TE?

I use a salting out method to isolate DNA from blood. Sometimes when I add TE to the participated DNA, it doesn't dissolve in it, even after one day. does anyone know why?

01 February 2014 3,018 4 View

Is it possible to isolate buffy coat from frozen blood?

We have some frozen blood in -20 freezer. They are there for about 1-2 months. Now I need to isolate WBCs. Does anyone know of an experiment I can use? I need them for DNA extraction.

01 February 2014 8,514 23 View

Does DNA get any damage if left dried without adding water or TE for some days after DNA extraction?

Will it be okay for experiments like PCR after dissolve in TE?

09 October 2013 7,718 6 View

I need to extract DNA from buccal swabs of babies, does anyone have an experience with some protocol, extraction kit or something?

If you have some experience or suggestion please let me know, any answer would be appreciated.

03 April 2013 8,427 3 View

I have to perform a PCR by a reverse primer which have a mismatch, one nucleotide before the 3' end. How could it affect my PCR?

Do I have a big problem?

11 December 2012 2,756 26 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View